The CellTiter-Glo® 2.0 Cell Viability Assay is a luminescent method for swift, convenient, routine cell viability assessments.
- Offers equivalent performance to the classic CellTiter-Glo® Cell Viability Assay
- ATP assay to identify living cells
- Shorter preparation times and easy repurposing over several days
New! Use with the New MyGlo Reagent Reader from Promega. A customized 96-well MyGlo reagent reader system incorporating instrument, assay preparation, and data processing will help users speed up and simplify cell viability tests. MyGlo detects more than just assay signals. It walks users through every step of the CellTiter-Glo® experiments.
CellTiter-Glo® 2.0 measures ATP, a key indicator of cell health
CellTiter-Glo® 2.0, built upon the foundational chemistry of the original CellTiter-Glo® Cell Viability Assay, features a single-reagent system with enhanced stability, simplifying storage and setup. Whether conducting cell proliferation assays on a small scale or handling numerous plates concurrently, this ready-to-use reagent streamlines daily operations. It is suitable for high-throughput screening and multiplex applications with its heightened sensitivity and wide linear range.
The CellTiter-Glo® 2.0 Assay gauges the number of viable cells in culture by measuring ATP levels as a marker for metabolically active cells. The luminescent signal obtained directly correlates with the abundance of viable cells in the culture.
Simple assay setup and enhanced stability
With a single, pre-prepared reagent and a straightforward "add-mix-measure" process, there is no requirement for mixing individual components.
The CellTiter-Glo® 2.0 reagent maintains stability both at room temperature and at 4 °C, facilitating rapid and effortless assay execution while eliminating the need for thawing and mixing, as required by alternative ATP assays. This enhanced stability also enables convenient storage.
Image Credit: Promega Corporation
Source: Promega Corporation
| Percent Activity Remaining |
CellTiter-Glo® 2.0 Reagent Stability |
CellTiter-Glo® "Original" Reagent Stability |
| >85% Activity at 22 °C |
7 days |
12 hours |
| >85% Activity at 4 °C |
2 months |
3.5 days |
Assay performance
The CellTiter-Glo® 2.0 Cell Viability Assay is appropriate for high-throughput screening and benchtop applications due to its superior performance compared to the original CellTiter-Glo® Assay.
High sensitivity and broad linearity. Luminescence measured with the CellTiter-Glo® 2.0 Assay is proportional to the number of viable Jurkat cells in culture over three orders of magnitude in 96-well, 384-well, and 1536-well plates. Image Credit: Promega Corporation
Multiplex with other cell viability or cytotoxicity assays
Multiplexing the CellTiter-Glo® 2.0 cell viability assay with other assays can decrease labor and costs associated with cell culture. Complementary measures of cell health can be used to compensate for mistakes in plating, culturing, or assay chemistry interferences by multiplexing assays in the same sample well.
In the provided example, bortezomib-treated K562 cells were exposed for 48 hours before the addition of CellTox™ Green cytotoxicity assay reagent. The fluorescence associated with cytotoxicity was then measured. The CellTiter-Glo® 2.0 assay reagent was introduced, and luminescence was measured to assess cell proliferation.
Image Credit: Promega Corporation
Specifications
What is in the box?
Source: Promega Corporation
| Item |
Part # |
Size |
| CellTiter-Glo® 2.0 Reagent |
G924A |
1 × 10ml |