CD19 proteins: Therapeutic targets for B-cell cancers

CD19, a B-cell surface protein, is expressed across B-cell development and it is expressed on almost all B-cell malignancies, such as ALL, chronic lymphocytic leukemia (CLL) and several non-Hodgkin lymphomas.

This universal expression and specificity for a single cell lineage have rendered CD19 an appealing target for antibody-based therapy, such as ADCs, bispecific antibodies, Fc-engineered antibodies and CAR-T cell therapy.

Until now, two anti-CD19 CAR-T cell therapies and one bispecific anti-CD19/CD3 antibody have received approval from the FDA for the treatment of hematologic B-cell malignancies. As per ClinicalTrials.gov, until May 7th, 2020, over 763 clinical trials have been launched.

To promote the development of CD19-targeted antibody-based therapy, ACROBiosystems has come up with an extensive range of CD19 protein products, such as biotinylated CD19, fluorescent-labeled CD19 and unconjugated CD19. Such proteins are ideal for antibody screening, immunization, antibody affinity measurement and detection of anti-CD19 CAR expression. The high activity of such proteins has been confirmed by ELISA, SPR and flow cytometry (FACS). Protocols are provided for free. This can help users save the development cycle.

ACROBiosystems has presented DMF for its recombinant CD19 to FDA and the DMF number has been filed as 034936. If DMF filed CD19 proteins from ACROBiosystems have been utilized in users’ drug development process, they can request that the company to offer DMF authorization to FDA in favor of submission or filing that users have made to the FDA.

Source: ACROBiosystems

Molecule Cat. No. Host Product Description Structure
CD19 CD9-HP2H3 HEK293 PE-Labeled Human CD19 (20-291) Protein, His
Tag (Site-specific conjugation)Hot
CD9-HF2H2 HEK293 FITC-Labeled Human CD19 (20-291) Protein, His Tag DMF FiledHot
CD9-HF251 HEK293 FITC-Labeled Human CD19 (20-291) Protein, Fc Tag DMF FiledHot
CD9-HF2H3 HEK293 FITC-Labeled Human CD19 (20-291) Protein, His Tag Star Staining
CD9-H8259 HEK293 Biotinylated Human CD19, Fc Tag, ultra
sensitivity (primary amine labeling) DMF Filed
CD9-H82F7 HEK293 Biotinylated Human CD19 (20-291) Protein, Fc, Avitag™Hot
CD9-H82E9 HEK293 Biotinylated Human CD19 (20-291) Protein, His, Avitag™ (SPR verified)Hot
MBS-K005   Human CD19-coupled Magnetic Beads  
CD9-H5251 HEK293 Human CD19 (20-291) Protein, Fc Tag, low endotoxin (Super affinity, MALS verified) DMF FiledHot
CD9-H52H2 HEK293 Human CD19 (20-291) Protein, His Tag DMF FiledHot
CD9-H5259 HEK293 Human CD19 (20-291) Protein, Fc Tag, low endotoxin
CD9-H5250 HEK293 Human CD19 (20-291) Protein, Llama IgG2b Fc Tag, low endotoxin
CD9-H5258 HEK293 Human CD19 (20-291) Protein, Mouse IgG2a Fc Tag
CD9-C5251 HEK293 Cynomolgus / Rhesus macaque CD19 (20-292) Protein, Fc Tag
CD9-C5221 HEK293 Cynomolgus / Rhesus macaque CD19 (20-292) Protein, His Tag

 

Product features

Detection of anti-CD19 CAR expression by FACS

1e6 of the anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human CD19 (20-291), His Tag (Cat. No. CD9-HP2H3). PE Streptavidin was used as negative control (QC tested).

1e6 of the anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human CD19 (20-291), His Tag (Cat. No. CD9-HP2H3). PE Streptavidin was used as negative control (QC tested). Image Credit: ACROBiosystems

High affinity verified by SPR

Biotinylated Human CD19 (20-291), His, Avitag (Cat. No. CD9-H82E9) captured on Biotin CAP - Series S sensor Chip can bind FMC63 MAb (mouse lgG2a) with an affinity constant of 0.255 nM as determined in an SPR assay (Biacore 8K) (QC tested).

Biotinylated Human CD19 (20-291), His, Avitag (Cat. No. CD9-H82E9) captured on Biotin CAP - Series S sensor Chip can bind FMC63 MAb (mouse lgG2a) with an affinity constant of 0.255 nM as determined in an SPR assay (Biacore 8K) (QC tested). Image Credit: ACROBiosystems

Higher binding affinity than competitive products verified by ELISA, FACS, and SPR

Binding activity of FITC-Labeled Human CD19, His Tag from two different vendors were evaluated in the ELISA analysis against FMC63 Mab. The result showed that ACRO’s FITC-Labeled Human CD19, His Tag has a much higher binding activity than that of the other vendor.

Binding activity of FITC-Labeled Human CD19, His Tag from two different vendors were evaluated in the ELISA analysis against FMC63 Mab. The result showed that ACRO’s FITC-Labeled Human CD19, His Tag has a much higher binding activity than that of the other vendor. Image Credit: ACROBiosystems

Binding activity of FITC-Labeled Human CD19, His Tag from two different vendors were evaluated in the flow cytometry analysis against anti-CD19-CAR-293 cells. The result showed that ACRO

Binding activity of FITC-Labeled Human CD19, His Tag from two different vendors were evaluated in the flow cytometry analysis against anti-CD19-CAR-293 cells. The result showed that ACRO's FITC-Labeled Human CD19, His Tag has a much higher binding activity than that of the other vendor. Image Credit: ACROBiosystems

Binding activity of Human CD19, His Tag from two different vendors were evaluated by SPR assay against FMC63 MAb. The result showed that ACRO

Binding activity of Human CD19, His Tag from two different vendors were evaluated by SPR assay against FMC63 MAb. The result showed that ACRO's Human CD19, His Tag can bind FMC63 MAb with an affinity constant of 2.95 nM which is much higher than that of the other vendor. Image Credit: ACROBiosystems

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