This webinar presents the use of a handheld Raman analyzer with onboard chemometrics to verify the identify and monitor the quality and batch-to-batch variability of both dry powder chemically defined media and ready-to-use liquid cell culture media.
While scans of cell culture media using the Thermo Scientific™ TruScan™ RM handheld Raman analyzer produce spectra with good Raman features, due to similar constituents and formulations, the TruTools add-on chemometric software package is required to verify cell culture media specifically.
Formulation complexity or mixtures with similar components at different concentrations require additional chemometric algorithms to allow accurate identification. TruTools offers advanced preprocessing, qualitative discrimination, and a platform to deploy customized chemometric models, including PCA and PLS-DA, on board the handheld Raman device.
Using the Raman analyzer with onboard chemometrics, users can spectroscopically monitor the long-term storage of media and be alerted of changes due to degradation. This goes to the heart of understanding and controlling batch-to-batch variability of media due to incoming quality or aging.
About the speaker
This webcast features Dean Stuart a Product Manager for Thermo Fisher Scientific who is passionate about the advancement of scientific instrumentation and the adoption of new and emerging technologies for the life sciences.
Throughout his career, Dean has shown a special interest in sustainable quality improvements for the pharmaceutical industry. His prior roles include a quality control scientist and an analytical methods developer.
Dean currently specializes in the development of Process Raman, Handheld Raman, Near Infrared Spectroscopy (NIR), and X-ray Fluorescence (XRF) technologies.
Watch the recorded webcast below.
Cell Culture Media Analysis Using a Handheld Raman Analyzer with Onboard Chemometrics