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Forensic Epigenetics, A Novel Method For Body Fluid Identification and Phenotyping

From Feb 28 2018

Diagnostic epigenetic loci are typically located using whole genome array studies which are then followed by quantitative pyrosequencing.

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Because methylation information is lost following the PCR, extracted samples are subjected to bisulfite modification which converts unmethlated cytosines into thymines following PCR. Pyrosequencing is next used to provide a quantitative estimate of the level or percent methylation at each CpG site.

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