The clinical translation of human pluripotent stem cells (hPSCs) necessitates advanced approaches to ensure their safe and reliable long-term growth and functional differentiation.
The development of a novel small molecule cocktail was recently reported. This cocktail can be utilized as a universal end-to-end solution, facilitating stress-free culture of human cells.
This four-part small molecule cocktail, termed CEPT (Chroman 1, Emricasan, trans-ISRIB, Polyamines), enhances the cell survival of genetically stable hPSCs. This is achieved by concurrent blockage of numerous stress mechanisms that would otherwise compromise cell structure and function.
CEPT improves routine cell passaging, embryoid body and organoid formation, cryopreservation/thawing of pluripotent and differentiated cells, single-cell cloning, genome editing, and new iPSC line generation.
CEPT, therefore, represents a distinct poly-pharmacological approach for comprehensive cytoprotection, offering a novel rationale for the effective and safe usage of hPSCs. The use of multi-target drug combinations to confer cell fitness may become a common method in cryobiology, regenerative medicine, and drug development.
What you will learn
- Easy and efficient techniques for isolating individual cells for downstream applications
- How to improve cell survival of genetically stable hPSCs
- How to guarantee safe and reliable stem cell growth with an innovative, four-part small molecule cocktail