RNA sequencing is a powerful tool that helps researchers uncover novel biological insights into the transcriptome.
This free eBook allows readers to learn about RNA sequencing (RNA-Seq) by explaining what it is, why it is used and how it works.
It also provides useful information regarding this popular next-generation sequencing technique to aid researchers in selecting the best method to achieve optimal results for their RNA-Seq experiments.
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The topics covered include:
- The science behind RNA-Seq: Explore the anatomy of the transcriptome and discover the information RNA-Seq can provide to help researchers attain novel biological insights.
- Technologies: Explore the most common RNA-Seq techniques and platforms to determine which is the most suitable for certain objectives, starting materials, and more.
- Workflows: Understand the five steps of a typical RNA-Seq project workflow, including design, extraction, library preparation, sequencing, and data analysis.
- Tips for designing an experiment: Learn how to set up your project for success with questions to consider when designing your RNA-Seq experiment, including resources for bioinformatic data analysis.
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