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Organoid Production Methodology

One of the ways to understand tissue organization and function is to recreate it ex vivo. This kind of experiment can provide insights into what kinds of cells and signaling is critical for the development and function of a tissue or organ. This attempt to create tissues and organs outside the body led to the creation of organoids which are self-organized, three-dimensional tissues, although they mostly represent partial regions of a tissue.

28 Jun 2018

Bioorthogonal Chemical Reporters

Bioorthogonal chemical reporters are small exogenous reporters which do not chemically modify the biomolecules they are tagged to.

27 Jun 2018

ChIP-Seq Analysis: Histone Modifications

ChIP-Seq is a powerful tool that enables researchers to investigate and understand protein−DNA interactions and the influence these have on gene expression and cell function.

27 Jun 2018

DNA Loop Extrusion Mechanisms

DNA loop extrusion is the formation of DNA loops when the DNA slips through ring-like motor proteins. Many theories have been proposed to explain loop extrusion.

27 Jun 2018

Structured Illumination Microscopy (SIM)

Structured Illumination Microscopy (SIM) is a tool which breaks the diffraction resolution barrier to provide resolutions of up to 20nm.

27 Jun 2018

CRISPR Interference (CRISPRi) for Gene Suppression

CRISPR (cluster of regularly interspaced palindromic repeats) are sequences found in approximately 50% of bacteria and approximately 90% of archaea, providing adaptive immunity against viral infection. Using these sequences, scientists have recently developed a new genetic technique called CRISPR interference (CRISPRi), which allows the suppression of genetic transcription.

27 Jun 2018

Lipidomics in Research

The complete set of lipids in an organism or a cell along with its interactions with other molecules, such as other lipids, proteins, and metabolites constitutes the lipidome. Lipidomics is the comprehensive and quantitative study of the lipidome. It involves identification and quantitation of thousands of biological pathways involving lipids and their interactions.

27 Jun 2018

Nanobody Applications

Nanobodies are therapeutic proteins derived from the heavy chain domains of IgG of camels. Usually antibodies have two identical heavy (H) and light (L) polypeptide chains. However, camel species have a unique form of IgG that has no L chains.

26 Jun 2018

What is Dynein?

Dynein, one of three cytoskeletal motor protein families, was first identified a half century ago and got its name after the ‘dyne’ (i.e. a unit of force).

26 Jun 2018

Metabolomics in Pharmacology

Metabolomics is the holistic study of metabolism. The application of metabolomics in pharmacology gives rise to a new field called “pharmacometabolomics.” The basic tenet of pharmacometabolomics is to determine the effects of drug treatment on the body’s metabolic scenario. It is also used to identify specific metabolic pathways that are responsible for drug-mediated outcomes.

26 Jun 2018

What are Nanozymes?

Nanozymes are nanomaterials that display enzyme-like characteristics. They are an important way of connecting nanomaterials to biological systems.

25 Jun 2018

What is the Difference Between Coulometric and Volumetric Titration?

Karl Fischer titration is used to determine the water content of samples. It can be performed using either volumetric or coulometric measurement techniques.

25 Jun 2018

Enteroids in Research

Intestinal stem cells can be cultured in the laboratory to produce 3D structures called enteroids, which closely mimic the normal human intestine.

25 Jun 2018

Stochastic Optical Reconstruction Microscopy (STORM)

Stochastic Optical Reconstruction Microscopy (STORM) is the one of the most widely used forms of super-resolution microscopy.

21 Jun 2018

The i-motif in DNA

Cytosine rich DNA sequences can fold into a structure called an i-motif. These structures typically appear in non-coding or promoter regions on DNA.

21 Jun 2018

What is Proteostasis?

Proteostasis is a complex pathway that functions to maintain all of the proteins within and around a cell.

21 Jun 2018

The IDO Pathway

The enzyme Indoleamine 2,3 dioxygenase (IDO) is part of a metabolic pathway involved in inflammation.

21 Jun 2018

In-Cell NMR

In-cell NMR is used to study the structure of molecules and metabolites inside a cell at an atomic resolution.

20 Jun 2018

Nanobody Development

Nanobodies are therapeutic proteins derived from the heavy chain domains of IgG of camelids. They can be used for oral immunotherapy, dentistry and sepsis.

20 Jun 2018

Anti-Counterfeiting Technology for Medical Devices

The WHO estimates that 8 percent of medical devices are counterfeit. There are, however, measures that can be taken to protect real devices and detect fakes.

20 Jun 2018