New clues to pancreatic cells' destruction in diabetes

Feb 3 2009

Researchers have found what appears to be a major culprit behind the loss of insulin-producing beta cells from the pancreases of people with diabetes, a critical event in the progression of the disease.

The discovery could lead to new therapies for preventing the death of beta cells or restoring those that have already been lost, Kathrin Maedler and colleagues report in the February 4th issue of Cell Metabolism , a Cell Press publication. The inflammatory factor they uncovered, which they call CXCL10, might also offer a warning sign of early or impending disease, they said.

"Previously, the idea was that insulin resistance makes one diabetic, but loss of beta cells occurs in both type 1 and type 2 diabetes," Maedler said, noting that among those who are insulin resistant, only 10-20 percent will go on to develop type 2 diabetes due to a failure of beta cells. "We've found an inflammatory marker for both types of diabetes. If we can protect cells from CXCL10 expression, we might prevent the decline in beta cell mass and, with it, the disease."

Type 1 diabetes is usually diagnosed in children or young adults and stems from an inability to produce insulin. The more common type 2 diabetes generally arises later in life when the body fails to produce enough insulin or grows unresponsive to the hormone.

In type 1 diabetes, beta cells are known to be destroyed by the immune system and its production of high concentrations of inflammatory signals. While scientists had floated many ideas, exactly what causes beta cell loss in type 2 diabetes remains a matter of debate.

Maedler's team suspected that inflammatory factors might play a key role there as well. Indeed, inflammatory markers are found in obesity, insulin resistance and diabetes, they explained. Earlier studies also showed that low-grade inflammation and activation of the innate immune system-the body's first line of defense--can lead to beta cell failure in type II diabetes.

They've now found that the inflammatory factor CXCL10 (also known as Interferon-gamma-inducible Protein-10, or IP-10) is an important trigger for beta cells' destruction. They found that hormone-producing cells isolated from patients with type 2 diabetes secrete CXCL10 and contain more than 30 times the amount of the CXCL10 message in the form of RNA than do cells from patients without diabetes.

Pancreatic sections taken from obese people without diabetes as well as those with type 1 or type 2 diabetes showed CXCL10 in the beta cells, they found. Moreover, treatment of isolated human pancreatic cells with CXCL10 decreased beta cell viability and impaired the production and secretion of insulin. They traced those effects of CXCL10 to a well-known pathway of the innate immune system involving a protein known as toll-like receptor 4 (TLR4).

The new data suggest a potential mechanism for the switch from beta cells' proliferation to their programmed cell death, the researchers concluded. "To prevent such a progression using anti-inflammatory targets of the TLR4 signaling pathway will be of high importance to rescue the beta cell from inflammation-induced self-destruction and [to] preserve beta cell function and mass."