Bruker announces world's first 1.2 GHz high-resolution protein NMR data

Bruker Corporation today announced the world’s first 1.2 GHz high-resolution, protein nuclear magnetic resonance (NMR) data at EUROISMAR 2019.

Two 1.2 GHz superconducting magnets have now reached full field at Bruker’s Swiss magnet factory, setting the world record for stable, homogeneous NMR magnets for high-resolution and solid-state protein NMR applications in structural biology and for the study of intrinsically disordered proteins (IDPs).

1.2 GHz 1H-15N 2D BEST-TROSY (left) and 2D plane of a 1.2 GHz 3D 15N edited NOESY-HSQC of a 500 µM ubiquitin sample, 13C/15N labeled in H2O:D2O 90%:10%. Both experiments were recorded with a 3 mm TCI CryoProbe.

At EUROISMAR 2019, Bruker and its scientific collaborators are presenting 1.2 GHz high-resolution NMR data that has been acquired using a new 1.2 GHz 3 mm triple-inverse TCI CryoProbe.

Bruker’s unique 1.2 GHz ultra-high field NMR magnets utilize a novel hybrid design with high-temperature superconductor (HTS) inserts inside advanced, low-temperature superconductor (LTS) outserts, which together provide the extremely demanding stability and homogeneity for high-resolution protein NMR.

Professors Lucia Banci and Claudio Luchinat at the University of Florence, Italy, are expected to be the first customers to receive a 1.2 GHz NMR spectrometer, once further systems development and factory testing has been completed, a process that is expected to take several more months. After initial data acquisition of CERM test samples on one of the 1.2 GHz systems, they stated:

"At Bruker's UHF facility in Switzerland, high resolution spectra have been acquired on alpha-synuclein, which is an intrinsically disordered protein that has been linked to diseases such as Alzheimer's and Parkinson's. In addition, we have also been able to review first 1.2 GHz NMR spectra of a protein which is associated with several types of cancer.

!Without a doubt, the improved resolution of the 1.2 GHz instrument - made possible by the increased dispersion at high magnetic fields - will help to advance important fields of research, such as structural biology. We look forward to receiving the 1.2 GHz NMR spectrometer in our laboratory once final developments and factory evaluation have been completed."

The new 1.2 GHz system is a technological revolution that will enable new molecular and cell biology discoveries. We value the trust our ultra-high field NMR customers place in us, and we are proud to have achieved this further milestone of generating the world’s first high-resolution protein NMR data at 1.2 GHz.

While we are not yet completely finished with all developments for the new 1.2 GHz system, our recent rapid progress is a testament to our commitment to innovation, and to working collaboratively with our customers to develop enabling scientific capabilities.”

Dr. Falko Busse, Group President of Bruker BioSpin

Similar to the previously announced Ascend 1.1 GHz magnet, the Ascend 1.2 GHz hybrid HTS/LTS magnet is a standard-bore (54 mm), two-story magnet system with drift and homogeneity specifications similar to Bruker’s existing 900 MHz and 1 GHz ultra-high field NMR magnets, ensuring compatibility with a range of NMR probe types and spectrometer accessories.

Bruker’s Ascend™ 1.2 GHz NMR magnets leverage the same advanced conductor and magnet technologies for winding, jointing, force management, quench protection, low drift and high homogeneity that were developed successfully for the Ascend 1.1 GHz magnet that was announced as a product at ENC 2019.