BioRescue research project exemplifies challenges posed by global pandemic

The BioRescue research project, a program aiming at saving the northern white rhinoceros from extinction, exemplifies the challenges to overcome when conducting research and conservation in an international consortium in times of a global pandemic.

COVID-19 hampered communication and travels, prevented or delayed crucial procedures, caused losses in revenues, and by that may have lowered the chances of survival of the northern white rhino. The consortium adjusted strategies gained valuable knowledge during these challenging times and continued with its mission.

The effects of the pandemic on the BioRescue project are described in detail in a scientific paper published in the Journal of Applied Animal Ethics Research.

There are only two northern white rhino individuals left in the world, both females. To prevent the extinction of the northern white rhino, an international consortium of scientists and conservationists seeks to advance assisted reproduction technologies and stem-cell associated techniques to create northern white rhino embryos in-vitro.

In the near future, the embryos will be transferred to southern white rhino surrogate mothers to create northern white rhino offspring. This boundary-pushing program is conducted by an international team working within a global framework and includes scientists and conservationists from institutions in Germany, Kenya, Japan, Czech Republic, USA, and Italy.

From March 2020 onwards, the work of the consortium has been severely affected by the Covid-19 pandemic in several ways at the local, national and international levels.

At the international level, the most striking obstacle was international travel restrictions. "The consortium partners had previously agreed upon collecting oocytes from the last two northern white rhinos every three to four months. This is considered a safe interval to maintain the health of the females while maximizing the number of harvested oocytes, equivalent to potential future embryos and offspring" says BioRescue project head Thomas Hildebrandt from the Leibniz-Institute for Zoo and Wildlife Research (Leibniz-IZW).

Following such an interval, oocyte collection was planned for March 2020 at the Ol Pejeta Conservancy in Kenya.

"Owing to international travel restrictions, the procedure had to be canceled and could only be conducted after the re-opening of Kenya's borders in August 2020," adds Leibniz-IZW BioRescue scientist Susanne Holtze, who shares the first authorship of the publication with Hildebrandt.

"This does not only mean that one crucial opportunity was missed and possibly several valuable embryos could not be generated, but it also affected the subsequent procedure in August 2020," Holtze explains.

It is likely that the prolonged interval since the last oocyte collection in December 2019 compromised oocyte quality and was the reason that out of 10 oocytes, no embryos could be created. The delay of possible embryo transfers in Kenya will also decrease the chances for northern white rhino calves to grow up with individuals of their kind.

This ultimately implies that almost a year was lost for the program - a serious delay in the race against time to prevent the extinction of the northern white rhino.

On the other hand, the involuntary break provided us with valuable new insights into the reproduction management of northern white rhinos. We still made progress in 2020 as we could successfully continue our research with our Kenya mission in December."

Thomas Hildebrandt, BioRescue Project Head, Leibniz-Institute for Zoo and Wildlife Research (Leibniz-IZW)

In addition to the delays in conducting the procedures at Ol Pejeta Conservancy in Kenya, for which strict travel regulations and on-site hygiene rules had to be followed, the pandemic posed several further challenges to the consortium. Lockdown and temporal closures of public facilities caused notable losses in revenues for the consortium partner Safari Park Dv?r Králové in the Czech Republic.

"We faced the unprecedented situation of having no revenues from entrance fees and other services. However, against all odds, we were quickly able to develop new ways of how to approach our potential visitors and supporters online and this allowed us to keep our support to the northern white rescue program on the same level as in previous years. Our highest priority is protecting species from extinction and COVID-19 confirmed how important the support by individual donors is," says Jan Stejskal, the Safari Park's Director of Communication and International Projects.

Similarly, the not-for-profit Ol Pejeta Conservancy experienced drastic reductions in revenue from international tourism owing to a ban on international travel, national curfews, and the isolation of the capital Nairobi.

"Therefore, fundraising was necessary to maintain our wildlife and conservation programs and pay for salaries," says Ol Pejeta Managing Director, Richard Vigne. "Nevertheless, safeguarding the animals and professional veterinary care was maintained at all times in cooperation with the Kenya Wildlife Service."

For the research facilities of the consortium partners in Germany, Italy, and Japan, different levels of restrictions were put in place on laboratory work. Crucial work at Leibniz-IZW, Avantea Laboratory of Reproductive Technologies (Italy), Kyushu University (Japan), and the Max Delbrück Center for Molecular Medicine (Germany) could be carried on to a limited extent.

"Staff at labs was limited, hygiene rules were enacted, transportation of samples and equipment were hampered and last but not least closure of schools and child care facilities forced parents to switch from lab work to mobile work at home," Sebastian Diecke from Max Delbrück Center sums up. Laboratories and offices at the University of Padova were also closed and online-teaching and research were implemented.

The team in charge of the ethical monitoring of the BioRescue program continued working from home and had to adjust strategies to carry on the ethical assessment for all procedures. "Despite all difficulties, the ethical assessment was always performed and BioRescue procedures have uninterruptedly maintained high standards of quality and respect for the safety and welfare of both researchers and the animals involved," says Barbara de Mori from the University of Padua.

"COVID-19 has disastrous consequences all over the world, but two new embryos that we produced in December 2020 demonstrate that our BioRescue team is committed to overcome all scientific and logistic challenges the northern white rhino rescue might bring. We will be grateful for everyone who decides to support us in our mission," adds Jan Stejskal."

Besides the downside effects of the pandemic, there were also a few positive ones. For example, the closure of international borders opened up new opportunities for assisted reproduction procedures in Germany which were important for advancing and perfecting methods and techniques.

For example, a designated mating partner of a southern white rhino female in a German zoo could not be transferred, and therefore, assisted reproduction was a welcome alternative to regular natural mating. Secondly, social distancing regulations helped to establish a new culture of online meetings within the consortium partners on a more regular basis, which proved useful and will continue in the future.

Lastly, there is a renewed awareness for the destruction of habitat and the loss of biodiversity as key drivers for emerging zoonotic diseases.

"BioRescue is not only about saving the northern white rhino. In the long run, it also is a much-needed step for the healing of disrupted habitat in Central Africa and therefore for preventing global pandemics in future," says Thomas Hildebrandt. "It is ironic and bitter, that our mission was severely affected by the very thing it ultimately intends to make more unlikely, a pandemic."