Quantitative polymerase chain reaction (qPCR) is a highly sensitive technique that allows researchers to amplify specific DNA sequences from a minimal starting amount.
However, this sensitivity poses a challenge in terms of potential contamination. Even the smallest quantities of DNA fragments from the laboratory environment or previous qPCR experiments can inadvertently undergo amplification during the reaction, leading to misleading outcomes like false positives. Identifying and resolving contamination issues can be particularly difficult for individuals new to qPCR experimentation.
Once DNA contamination takes place, it cannot be eliminated or reduced. Hence, prevention becomes paramount.
Whether you are looking to identify the origin of potential contamination or seeking to enhance the efficiency of your assay, this free guide from LGC Biosearch Technologies will help you assess and mitigate contamination in your qPCR experiments.
Get this whitepaper to help you:
- Map unexpected results to possible sources.
- Determine where your qPCR assay is vulnerable to contamination.
- Follow recommendations to mitigate identified vulnerabilities.
- Master the use of controls in routine testing.
Image Credit: LGC Biosearch Technologies
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