Single-cell isolation with cellenONE X1 NEO

cellenONE® is a comprehensive platform that seamlessly integrates high-accuracy single-cell isolation with precise pico- to nanoliter reagent dispensing.

  • Image-based single-cell sorting and isolation
  • Efficient cell line development with high clonal outgrowth
  • Miniaturized, end-to-end workflows for single-cell sample preparation

While cellenONE X1 NEO is the ultimate platform for single cell proteomics, it is compatible with any single cell applications.

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Benefits

Accuracy

  • Up to 100 % accuracy in single cells (no doublets, debris, or empty wells)

Miniaturization

  • Reagent dispensing with high precision (from pico to nanoliter)
  • On-deck temperature, humidity, and dew-point control

Image-based technology

  • Live imaging of cell sample content (e.g., diameter and elongation)
  • Sorting parameters can be user-defined (based on morphology or fluorescence)
  • Image-based QC for each isolated cell

Versatility

  • Any cell type (from 0.5 to ~80 µm)
  • Any sample size (from ~ 3 µL)
  • Any standard (i.e., 96, 384, 1536 well plates, glass slides, etc.) or custom labware

Viability

  • Ultra-gentle, acoustic-based dispensing technology
  • On-deck sample temperature control for enhanced cell viability (NEW)

Image of the cellenONE®.

Image Credit: SCIENION GmbH

Automation

  • Enhanced drop generation system (NEW)
  • Fully automated run creation and error handling (NEW)
  • Rapid temperature ramping (4–85 °C) ensures effective sample incubation (NEW)

Ideal for rare cells

  • Compatible with samples containing very few cells (e.g., micro-biopsies, CSF fluid)
  • Supports ultra-low sample volumes (starting from ~3 µL)
  • Maximized cell recovery—non-isolated cells are collected for further reprocessing

Time and cost savings

  • Isolates 96 cells in under 3 minutes
  • Miniaturized workflow to reduce reagent costs
  • Automated workflow to minimize operator time

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Applications

Single-cell isolation with cellenONE X1 NEO

Image Credit: Cellenion

 

How to isolate single cells with cellenONE from cellenion

Video Credit: Cellenion

Product specifications

Source: Cellenion

. .
Sorting Technology Brightfield and/or Multi-fluorescence
(Blue, Green, Orange and Red) images

DAPI (ex. 375 nm; em. 432 nm)
FITC (ex. 470 nm; em. 515 nm)
Cy3 (ex. 565 nm; em. 580 nm)
Cy5 (ex. 625 nm; em. 670 nm)
Dispensing technology sciDROP PICO: 250-600 pL / single drop
Target capacity 2 MTPs or 8 standard glass slides
Axis system X-Y Linear Magnetic Drive, Z Spindle Drive
Axis precision < 3 μm
Dimensions with enclosure
(L x Z x H)
650 x 700 x 1590 mm
Weight Approx. 242 kg

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