Polymers show promise for gene delivery

Apr 3 2007

Virginia Tech polymer scientists have developed a new family of gene vectors , novel polymers that can ferry genetic material across the cell membrane so that it can be incorporated into the machinery of the cell.

Representing Virginia Tech faculty members and students from engineering, chemistry, and veterinary medicine, Chemistry Professor Tim Long will give an invited lecture at the 233rd National Meeting of the American Chemical Society in Chicago March 25-29.

The presentation will be an overview of novel polymers developed by Virginia Tech researchers for biomedical applications, with an emphasis on gene delivery and tissue scaffolds. "Both of these emerging technologies are enabled with fundamental advances in polymer chemistry," Long said.

'synthetic macromolecules can be easily modified to contain a variety of functional elements capable of interacting with biological systems," he said. "Initial studies have found macromolecular topology to be a significant parameter in the delivery of DNA into cells."

In the cell, the new DNA initiates the manufacture of therapeutic proteins, such as might be needed to treat a genetic disease where an enzyme or protein is not produced naturally. The Virginia Tech vectors presently being tested in cell cultures are proving to be superior to surfactant benchmarks and offer reduced toxicity to viral vectors, Long said.

Meanwhile, scientists at Virginia Tech have developed a single-step process for creating fibrous mats from a small organic molecule , a new nanoscale, biocompatible material (Jan. 20, 2006, Science, "Phospholipid Nonwoven Electrospun Membranes," by Matthew G. McKee, John M. Layman, Matthew P. Cashion, and. Long, all at Virginia Tech.).

Since last year, they have improved the durability of the phospholipids through novel photochemistry during electrospinning and have begun to impregnate the porous mats with cells that will initiate tissue regeneration.

Long will present the talk, "Macromolecules with tailored non-covalent interactions for biomedical applications, (PMSE 198), at 2 p.m. Tuesday, March 27, in McCormick Place South room S505B. Authors are chemistry graduate students John M. Layman of Richmond, Va., and Matthew T. Hunley of Aiken, S.C., Wake Forest-Virginia Tech School of Biomedical Engineering (SBES) doctoral student Anjali A. Hirani of Orlando, Fla., and her advisor, SBES assistant professor Yong Woo Lee, Virginia-Maryland Regional College of Veterinary Medicine PhD candidate Benjamin Lepene of Franklin, N.H., and his advisor, Large Animal Clinical Sciences Professor Craig D. Thatcher, and Long.

The research is supported by the Macromolecular Interfaces with Life Sciences (MILES) National Science Foundation Integrative Graduate Education and Research Traineeship (IGERT) program and the U.S. Army Research Office Multidisciplinary University Research Initiative (MURI), which brings together chemistry, mechanical engineering, electrical engineering, chemical engineering, and materials science researchers to accelerate discoveries in nanostructured materials