Study shows WATS3D biopsy can help overcome limitations of histopathological assessment of esophageal precancer

Sep 14 2015

New Study Results Published in the American Journal of Gastroenterology

Prevention of Esophageal Adenocarcinoma, the most rapidly growing cancer in the US and one of the most fatal, relies upon the detection of precancerous cells sampled during an endoscopy. This has historically been hampered by low inter-observer agreement among pathologists when examining esophageal tissue samples using a standard manual microscope. CDx Diagnostics today announced results from a study examining inter-observer agreement rates among pathologists using the WATS^3D three dimensional computer-assisted biopsy system. The study, published in the September issue of the American Journal of Gastroenterology, found that the diagnosis of Barrett's esophagus and associated dysplasia using WATS^3D has a rate of inter-observer agreement that is significantly higher than published estimates for standard manual microscopic assessment.

The WATS^3D biopsy collects a wide area, disaggregated tissue specimen of the entire thickness of the suspect epithelium resulting in a 100 micron thick smear. Standard biopsies are mechanically cut into thin, 2-3 micron slices in order not to exceed the very narrow depth of field of a standard manual microscope. This century-old process alters much of the natural appearance of the tissue and can deprive the pathologist of potentially important diagnostic information. In a manner somewhat analogous to how a CAT scan combines multiple two dimensional x-rays to form a single three dimensional image, the WATS^3D computer integrates over 100, one micron thick optical slices to form a single three dimensional image in which potential abnormality is highlighted to the pathologist.

"This study addresses a major clinical challenge in gastroenterology," said Prateek Sharma, MD, Division of Gastroenterology and Hepatology, University of Kansas School of Medicine, Kansas City, MO. "The high rates of inter-observer variability among pathologists evaluating Barrett's esophagus complicate diagnosis and risk stratification and can confound the gastroenterologist's ability to make effective treatment decisions for the patient."

The histopathological diagnosis of Barrett's esophagus-associated dysplasia has poor inter-observer agreement even among experienced gastrointestinal pathologists. Published estimates for the inter-observer agreement ("kappa values") in the reading of dysplasia in Barrett's esophagus have varied between 0.15 and 0.69. Kappa values of 0.41 to 0.60 indicate moderate agreement, 0.61 to 0.80 indicate substantial agreement, and above 0.80 indicate nearly perfect agreement (Landis and Koch scale).

In the study evaluating inter-observer agreement with WATS3D, a total of 149 Barrett's esophagus slides were evaluated in a blinded manner by four pathologists. The slides included the following: no dysplasia (n=109), low-grade dysplasia (n=18), and high-grade dysplasia or esophageal adenocarcinoma (n=22).

The overall mean kappa value for all three diagnoses for the four observers was calculated at 0.86 (95% confidence interval (CI) 0.75-0.97). The kappa values (95% CI) for high-grade dysplasia or esophageal adenocarcinoma, indefinite or low-grade dysplasia, and no dysplasia were 0.95 (0.88-0.99), 0.74 (0.61-0.85), and 0.88 (0.81-0.94), respectively. Inter-observer agreement between any two pathologists ranged between 0.83 and 0.89. Percentage agreement was calculated at 88.6% (95% CI: 82.36-93.21) (132/149) among four pathologists.

"These data suggest the WATS^3D biopsy can help overcome the limitations of traditional histopathological assessment of esophageal precancer," said lead author Prashanth R. Vennalaganti, MD, Division of Gastroenterology and Hepatology, University of Kansas School of Medicine, Kansas City, MO. "WATS^3D uses a computer neural network that chooses the 200 most suspicious areas from the biopsy and projects the same cells on a computer monitor to every pathologist evaluating the sample. This may potentially explain the high inter-observer agreement observed in the study."

"One of the most meaningful findings in this study is the high inter-observer agreement for the diagnosis of low-grade dysplasia, which has historically had the lowest rate of agreement even among expert pathologists," said Lauren Gerson MD, MSc, Associate Clinical Professor of Medicine, University of California San Francisco. "Improved detection of low grade dysplasia will allow more gastroenterologists to change their surveillance protocols or perform endoscopic interventions for this patient cohort."

WATS^3D has been commercially available in the United States since 2012.