New optimising ChIP sequencing library preparation guide

Chromatrap®, a business unit of Porvair Sciences, has produced an informative new guide entitled ‘Top 10 Tips for ChIP-seq Library Preparation'.

ChIP-sequencing, also known as ChIP-seq, is a method used primarily to determine how transcription factors and other chromatin-associated proteins influence phenotype-affecting mechanisms. Determining how proteins interact with DNA to regulate gene expression is essential for fully understanding many biological processes and disease states. ChIP-seq combines chromatin immunoprecipitation (ChIP) with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins.

High quality library preparation is key to a successful ChIP-seq experiment. Written by scientists with many years’ experience of ChIP assay development, the new document provides an informative 10-point illustrated guide to help you improve your ChIP-seq library preparation.

Chromatrap’s® new ChIP SEQ kit utilises the solid state technology in parallel with high throughput sequencing to deliver a streamlined ChIP-seq protocol from small cell numbers and low chromatin concentrations. Specifically adapted for broader chromatin concentrations, Chromatrap® ChIP-seq now combines the dynamic range of Chromatrap® with the downstream analysis power of deep sequencing. This allows faster, more reproducible genome wide identification of TF binding sites and specific DNA associated protein modifications. With no limitation in scale or resolution, NGS can elucidate the role of TFs and epigenetic marks on gene transcription and epigenetic chromatin status.

To download a pdf version, or request a hard copy, of this new guide please visit:

• https://www.chromatrap.com/

​or contact the Chromatrap® customer services team on:

• +44-1978-666222
• [email protected].

Chromatrap® is a business unit of Porvair Sciences, a wholly owned subsidiary of Porvair plc.