Dec 9 2016
IDT demonstrates how ribonucleoprotein delivery overcomes challenges in CRISPR-mediated genome editing
Integrated DNA Technologies (IDT) will host an informative webinar entitled ‘Ribonucleoprotein delivery of CRISPR-Cas9 reagents for increased gene editing efficiency’ on December 14, 2016.
The webinar, by Dr Rolf Turk, Research Scientist, Molecular Genetics at IDT, will be held at two different times (9am and 1pm Central Standard Time) and will describe the advantages of using a Cas9:crRNA:tracrRNA ribonucleoprotein (RNP) complex for increased efficiency in genome editing.
The CRISPR/Cas9 system is now one of the most well-known tools for effective genome modifications, but there are still common issues with low editing efficiency or delivery problems in primary or difficult-to-transfect cells. This webinar will describe the benefits of using RNP complexes for efficient and effective delivery, including ease of use, limiting off-target effects, and stability.
Dr Rolf Turk, who has been working at IDT to optimize delivery protocols for CRISPR, will also present data showing how genome editing efficiency rates are improved by IDT’s Cas9 electroporation enhancer, and provide advice on how to optimize transfection using the Alt-R™ CRISPR-Cas9 System in combination with different electroporation methodologies.
With various CRISPR methodologies to choose from, the webinar aims to assist attendees in selecting the most effective methods and tools for success in their genome modifications.
To register please select the desired session below, or contact IDT via the website www.idtdna.com.
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