Study shows how SARS-CoV-2 affects cytokines compared to other respiratory viruses

Respiratory viruses such as SARS-CoV-2 (causing COVID-19) can often catalyze an overactive immune response that leads to a life-threatening cycle, known as a cytokine storm.

Analyzing cytokine responses from patients infected with SARS-CoV-2 and similar common respiratory viruses has unearthed glaringly important differences in how SARS-CoV-2 affects cytokines compared to other common respiratory viruses.

The comprehensive data resource aims to help specialists identify better treatments and diagnosis of underlying causes that can cause the deadly cytokine storm.

Scientists at the Earlham Institute (EI) and the Quadram Institute study how the immune system responds to infection with SARS-CoV-2 and other similar respiratory viruses, in particular to identifying unique features in severely ill COVID-19 patients.

Members of the Korcsmaros Group working alongside the clinical virologist Claire Shannon-Lowe at the University of Birmingham focused their attention on how SARS-CoV-2 and other respiratory viruses are causing the so-called 'cytokine storm' - a hyper-activation of our own immune system - one of the main reasons for the high death rate in a subgroup of COVID-19 patients.

To identify the similarities and differences in the cytokine storm, the researchers collected and analyzed the vast collection of thousands of COVID-19 research papers. They looked for patterns of cytokine changes in patients who had been infected by respiratory viruses that cause cytokine release syndrome.

By systematically analyzing over 5,000 scientific studies to find those containing immune response data from patients, the researchers showed that SARS-CoV-2 has a unique tendency of halting the rise of specific cytokines in certain patients when compared to other similar viruses. This is important in understanding the causes of the potentially fatal cytokine release syndrome, more commonly known as a cytokine storm.

Transmission electron micrograph of a SARS-CoV-2 virus particle (UK B.1.1.7 variant), isolated from a patient sample and cultivated in cell culture. The prominent projections (green) seen on the outside of the virus particle (yellow) are spike proteins. This fringe of proteins enables the virus to attach to and infect host cells and then replicate. Image captured at the NIAID Integrated Research Facility (IRF) in Fort Detrick, Maryland. Credit: NIAID

"As the onset of the cytokine storm is one of the key factors behind the mortality rates we're seeing in a particular group of COVID-19 patients, it is critical to understand why it is happening," said project lead PhD Student Marton Olbei in the Korcsmáros Group.

"Cytokine storms are not unique to SARS-CoV-2 infection; they can be found in most of the critical human coronaviruses and influenza. A subtype outbreaks of the past two decades."

Cytokines are small proteins that tightly regulate our immune system and how our body reacts to internal or external stress, such as cancer, inflammation, or infection. Cytokines act as conductors, orchestrating our immune response when infected with viruses. One of their roles is to cause inflammation, which is part of the healing process of many infections and injuries.

Respiratory viruses all activate antiviral responses in the body but there are differences in how each virus attempts to evade the attention of the immune system. The most common strategy is to confuse or specifically attack, crucial immune response mechanisms - such as the release of cytokines.

A cytokine storm happens only in certain patients' immune systems when reacting to a virus. A feedback loop causes the continual activation of cytokines responsible for inflammation, resulting in organ failure or even death.

While SARS-CoV-2 cases have distinct similarities to both influenza patients and those who were infected in previous coronavirus outbreaks (SARS-CoV, MERS-CoV), the researchers' analysis found specific immune mechanisms that make SARS-CoV-2 uniquely dangerous.

We examined the changing cytokine levels upon infection with similar viral pathogens (SARS-CoV, MERS-CoV, H5N1, H7N9) to highlight the protective and unique cytokine responses caused by these viruses."

Marton Olbei, PhD Student and Project Lead, Korcsmáros Group

By comparing the COVID-19 patients' immune response data, the researchers found similarities mounted against these pathogens - discriminating between influenza A subtypes and coronaviruses - and the unusual aspects of the current circulating SARS-CoV-2 virus.

SARS-CoV-2 is similar to other respiratory viruses but, by targeting specific regulators of the cytokine response, with just small-scale differences, it could lead to more severe disease - not from the virus itself, but from the patient's immune system response.

"For a subgroup of patients, when infected by these viruses, a real danger is posed by the immune system overreacting. We're drawing out which specific parts of our immune system react in a potentially harmful way to these viruses, said Marton.

"We wanted to take a step back and summarise what is actually being reported in the scientific literature, specifically focusing on cytokine-mediated immune responses, to put into context and differentiate SARS-CoV-2 from these other viruses. Building up a data repository such as this will also be vital for the future; if other similar viruses arose, you could quickly find their profile and compare."

A map of such immune responses could help clinical specialists identify interventions that have successfully alleviated cytokine storms in other diseases, and evaluate whether they could be effective in treating certain COVID-19 cases.