Study expands understanding of the eye's immune response to viral infections

Reviewed by Emily Henderson, B.Sc.May 24 2022

The cornea - the transparent protective outer layer of the eye critical to helping us see – produces a delicate and limited immune response to fight infections without damaging our vision, according to a ground-breaking new study from the Peter Doherty Institute for Infection and Immunity (Doherty Institute).

Published today in Cell Reports, the study has shown long-living memory T cells that patrol and fight viral infections are present in the cornea, upending current thought that T cells are not found in healthy corneas - expanding our understanding of the eye's immune response to infections.

The team used a multiphoton microscope that provides live images of living, intact biological tissues to study cornea cells in mice infected with Herpes Simplex Virus.

Their images revealed long-living memory T cells produced in the mice's eyes to fight the infection. The memory T cells remained in the cornea after the virus was eliminated to ward off any future reinfection.

Advanced imaging of the eye in healthy people also revealed immune cells patrolling the cornea – the first time that cells have been imaged moving in human eyes.

For humans to see, the cornea must remain transparent to ensure focused light is received by the retina through the iris.

The presence of T cells in the corneas was previously not considered, given the eyes produce only a dampened immune response to avoid inflammation that would obstruct our vision.

Lead author University of Melbourne Professor Scott Mueller, laboratory head at the Doherty Institute said that these discoveries have important implications for understanding how eyes ward off dangerous infections.

Current understanding that T cells are not found in healthy corneas needs to be reconsidered, as our discovery shows tissue-resident memory T cells entering the cornea and remaining there for long periods.

Our findings will improve the understanding of how to protect our eyes from infections that cause permanent blindness, such as Herpes Simplex Virus.

This also has implications for understanding chronic conditions such as dry eye disease and common eye allergies where unwanted T cells might also cause disease."

Professor Scott Mueller, University of Melbourne

This study was conducted in collaboration with the University of Melbourne's Department of Optometry and Vision Sciences and Department of Anatomy and Neuroscience, and Monash University's Institute of Pharmaceutical Sciences.