Mice study shows how working memory is maintained across interconnected brain regions

Reviewed by Danielle Ellis, B.Sc.Aug 3 2022

How does the brain keep in mind a phone number before dialing? Working memory is an essential component of cognition, allowing the brain to remember information temporarily and use it to guide future behavior. While many previous studies have revealed the involvement of several brain areas, until now it remained unclear as to how these multiple regions interact to represent and maintain working memory.

In a new study, published today in Nature, neuroscientists at the Sainsbury Wellcome Centre at UCL investigated the reciprocal interactions between two brain regions that represent visual working memory in mice. The team found that communication between these two loci of working memory, parietal cortex and premotor cortex, was co-dependent on instantaneous timescales.

There are many different types of working memory and over the past 40 years scientists have been trying to work out how these are represented in the brain. Sensory working memory in particular has been challenging to study, as during standard laboratory tasks many other processes are happening simultaneously, such as timing, motor preparation, and reward expectation.” 

Dr Ivan Voitov, First Author and Research Fellow, Mrsic-Flogel lab

To overcome this challenge, the SWC researchers compared a working memory-dependent task with a simpler working memory-independent task. In the working memory task, mice were given a sensory stimulus followed by a delay and then had to match the next stimulus to the one they saw prior to the delay. This meant that during the delay the mice needed a representation in their working memory of the first stimulus to succeed in the task and receive a reward. In contrast, in the working memory-independent task, the decision the mice made on the secondary stimulus was unrelated to the first stimulus.

By contrasting these two tasks, the researchers were able to observe the part of the neural activity that was dependent on working memory as opposed to the natural activity that was just related to the task environment. They found that most neural activity was unrelated to working memory, and instead working memory representations were embedded within ‘high-dimensional’ modes of activity, meaning that only small fluctuations around the mean firing of individual cells were together carrying the working memory information.

To understand how these representations are maintained in the brain, the neuroscientists used a technique called optogenetics to selectively silence parts of the brain during the delay period and observed the disruption to what the mice were remembering. Interestingly, they found that silencing working memory representations in either one of the parietal or premotor cortical areas led to similar deficits in the mice’s ability to remember the previous stimulus, implying that these representations were instantaneously co-dependent on each other during the delay.

To test this hypothesis, the researchers disrupted one area while recording the activity that was being communicated back to it by the other area. When they disrupted parietal cortex, the activity that was being communicated by premotor cortex to parietal cortex was largely unchanged in terms of average activity. However, the representation of working memory activity specifically was disrupted. This was also true in the reverse experiment, when they disrupted premotor cortex and looked at parietal cortex and also observed working memory-specific disruption of cortical-cortical communication.

“By recording from and manipulating long-range circuits in the cerebral cortex, we uncovered that working memory resides within co-dependent activity patterns in cortical areas that are interconnected, thereby maintaining working memory through instantaneous reciprocal communication,” said Professor Tom Mrsic-Flogel, Director of the Sainsbury Wellcome Centre and co-author on the paper.

The next step for the researchers is to look for patterns of activity that are shared between these areas. They also plan to study more sophisticated working memory tasks that modulate the specific information that is being stored in working memory in addition to its strength. For this, the neuroscientists will use interleaved distractors containing sensory information that bias what the mouse thinks is the next target. Such experiments will allow them to develop a more nuanced understanding of working memory representations.

This research was funded by the Wellcome Trust, the Gatsby Charitable Foundation, and the University of Basel.