1. Improper use of HEK-293 without SV40 infection results in flawed conclusion
    Nicholas Morrish Nicholas Morrish United States says:
    July 2, 2021 at 4:55 PM

    This paper is flawed, as they used HEK-293 cells, rather than the typically used HEK-293T cells(human kidney cells infected with SV40). Previous research had shown that regular HEK-293 cells do not create an appropriate amplification level and also were flawed as the cells were able to fight off infections on their own. If they repeat this with HEK-293T cells, they will not get the same results. This just goes to show that many aren't understanding the COVID-19 is a complication due to SARS-COV-2 reacting with other RNA/DNA virus's that have been dormant in the host, and that those without such infections don't suffer major effects of SARS-COV-2.

    Reply
    • Source?
      Jason A. Jason A. Canada says:
      July 16, 2021 at 4:56 PM

      I would be very interested to read the research you’re basing your claim on.

      Reply
    • Seeking Source
      Mary Beth E Mary Beth E United States says:
      January 28, 2022 at 12:07 AM

      Nicholas,
      I too am interested in knowing your source.

      The full text Abstract shows HEK293 A549-hACE2-TMPRSS2 cells from Germany were used in the study [1]. There are many suppliers of human embryonic kidney 293 (HEK-293)-derived cell lines, specifically designed for COVID-19 studies. For example, InvivoGen's ACE2-(TMPRSS2) cells express high levels of the host receptor ACE2 alone, or in combination with the host protease TMPRSS2, at their cell surface. Thus, these cells are permissive to infection by SARS-CoV-2 and Spike-pseudotyped lentiviral particles. Of note, the addition of TMPRSS2 in A549 cells only significantly increases their infectivity [in-house data]. This cell line collection is ideal for screening small molecule inhibitors and neutralizing antibodies that aim to block the virus-host interaction and cellular signaling outcomes [2].

      293T cells are derived from the human embryonic kidney 293 cell line. 293T cells contain the simian virus 40 (SV40) large T-antigen, which facilitates replication of transfected plasmids containing the SV40 origin of replication. 293T/ACE2 cells are transfected to express ACE2, AND HAVE BEEN USED FOR MANY SARS-COV CELL-CELL FUSION AND PSEUDOVIRUS ENTRY INHIBITOR STUDIES [3]. All caps for emphasis. That 293T has been used for many studies does not mean they are the only cells that can or should be used.

      Source 1:  www.biorxiv.org/.../2021.03.19.435959v1.full
      Source 2:  https://www.invivogen.com/covid-19-related-cells
      Source3 :  https://covdb.stanford.edu/cells-list/

      Reply
The opinions expressed here are the views of the writer and do not necessarily reflect the views and opinions of News Medical.
Post a new comment
(Logout)
Post

Terms

While we only use edited and approved content for Azthena answers, it may on occasions provide incorrect responses. Please confirm any data provided with the related suppliers or authors. We do not provide medical advice, if you search for medical information you must always consult a medical professional before acting on any information provided.

Your questions, but not your email details will be shared with OpenAI and retained for 30 days in accordance with their privacy principles.

Please do not ask questions that use sensitive or confidential information.

Read the full Terms & Conditions.

Provide Feedback