Binding Kinetics is the foundation of modern drug design. This term refers to the speed at which a compound binds and dissociates with a target molecule.
Bio-layer interferometry (BLI) is an optical biosensing technology that analyzes interactions between biomolecules without fluorescent labels, which is one is its main advantages. It measures biomolecular interactions by analyzing the white light reflected from the tip of a biosensor.
This article will discuss the benefits of using biolayer interferometry (BLI) for measuring binding kinetics.
Which binding kinetics parameters can be determined using BLI?
One main advantage of biolayer interferometry over other binding kinetics measurement techniques is that it provides real-time determination of the association rate (kon) and dissociation rate (koff) of molecular interactions.
Determining these rates helps to calculate the affinity (KD.) between the two biomolecules. This value can be directly ascertained from the formation of concentration-dependent molecular complexes.
Benefits of BLI
BLI has several benefits for the measurement of binding kinetics. The main benefits are its simplicity, label-free nature, ability to determine a wide range of affinity values, and compatibility with a wide range of samples.
Simplicity
kon, koff, and KD can be rapidly measured in a single assay using BLI. Real-time detection without labeling biological molecules makes measurements much simpler and quicker than multiple-assay processes.
Wide ranges
A wide range of affinity values can be measured, making BLI ideal for determining binding kinetics. Many biomolecules, such as membrane proteins, petides, nucleic acids, lipid nanoparticles and AAVs, can be investigated using BLI.
Compatibility with a wide range of samples
BLI is compatible with crude and purified samples, without theneed to swap between systems ; the technique can be used in various investigations.
Label-free operation
Real-time response monitoring is facilitated by BLI’s label-free nature, improving the accessibility of kinetic information regarding association and dissociation. Interactions with native binding partners can be monitored without any impediment from modified analytes, producing more accurate biochemical data.
BLI for binding kinetics from Gator Bio
Gator Bio software provides a range of data analysis models for binding kinetics measurement and other critical drug design and biomedical research purposes. Reference subtraction, processing, and data fitting can be performed quickly.
Biosensors from Gator Bio combine a 1 mm diameter glass rod with patented optical layers and surface chemistry specifically built at the distal end of the biosensor. To find out more about how this technology can be used in binding kinetics applications, contact the team at Gator Bio today.
About Gator Bio, Inc.
Gator Bio is a world-leading biosensor company headquartered in Palo Alto, CA. At Gator Bio, we provide researchers the tools and instrumentation to advance their research. From antibody engineering to small molecule drug discovery to basic research, Gator Bio can be used to bring meaning to the unknown. From the original inventors of label-free biolayer interferometry (BLI), Gator Bio provides the next generation of BLI technology.
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