Typically, an antibody-drug, an anti-idiotype antibody (anti-ID Ab, see the schematic image) attaches itself to the idiotype of another antibody. An idiotype is the particular arrangement of idiotopes seen in complementary determining regions of an antibody.
Anti-ID Abs are frequently utilized in the preclinical setting to assess the pharmacokinetics (PK) and pharmacodynamics (PD) of antibody drugs because they can bind to them in biological fluids. Because of their similarities to anti-drug antibodies, anti-ID Abs are also frequently employed as a reference standard for antibody-drug immunogenicity, anti-drug antibody (ADA) studies, and neutralizing antibody measures.
GenScript collaborates with research to generate highly sensitive and specific polyclonal and monoclonal anti-ID antibodies tailored to specific downstream applications.
Highlights
- Fast turnaround time
- 7 weeks to deliver pure Anti-ID pAb
- 9 weeks to supply pure Anti-ID mAb
- High-quality delivery
- Antibody purity > 90%
- ELISA > 729K
- Cross-reaction < 2%
- Extensive expertise
- >20 years of global service experience
- > 90% success rate of anti-ID projects
Types of anti-idiotype antibodies
Source: GenScript
Type |
1. Antigen-blocking |
2. Non-blocking |
3. Complex-specific |
Schematic Diagram |
|
|
|
Feature |
Paratope-specific |
Not Paratope-specific |
Partially paratope-specific |
Antigen Binding |
Inhibitory |
Not inhibitory |
Not inhibitory |
Application |
Detect free drug only |
Detect total drug (free and bound) |
Detect bound drug typically |
Selection Guideline |
- Type 1 or type 2 could be applied to detect antibody drug when having a cell-bound drug target e.g. PD1/PDL1
- Type 1 & type 2 may both be needed when having a secret drug target e.g. TNF
|
Service details
Rabbit anti-ID monoclonal antibody package
Image Credit: GenScript
Customized Rabbit Anti-ID Monoclonal Antibody Package. Source: GenScript
|
Target Type |
Full length IgG/ADC/Bi-
specific Ab |
scFv/VHH |
RNA/VLP/(Neo)peptide /recombinant protein/small
molecule/Cell etc. |
Phase I
Immunogen Preparation |
F(ab’) 2 preparation or whole antibody/ADC |
Whole scFv/VHH |
KLH/BSA conjugation or
whole protein/cell |
Phase II
Animal Immunization |
Conventional immunization protocol
Express immunization protocol (MonoExpress™) Preferred |
Phase III
B Cell Cloning and Screening |
Indirect ELISA
Blocking ELISA* |
Phase IV
Sequencing & rAb
Production |
5 clones sequencing reports
Guaranteed 0.1 mg recombinant antibodies production and ELISA positive validation (With/without blocker & non-blocker) |
TAT |
Starting from 9 weeks |
* Required for blocking & non-blocker anti-ID screening
Rabbit anti-ID polyclonal antibody package
Image Credit: GenScript
Guaranteed Rabbit Anti-ID Polyclonal Antibody Package. Source: GenScript
|
Guaranteed Amount |
5/10/15/20 mg
>20 mg, please request a quote |
Target Type* |
Full-length IgG/ADC Bi-specific Ab |
scFv/VHH |
Phase I Immunogen Preparation |
F(ab’) 2 preparation or whole antibody/ADC |
scFv/VHH |
Phase II Animal Immunization |
Express immunization protocol (MonoExpress™)
with rabbits [OLAW&AAALAC-accredited facility] |
Express immunization protocol (MonoExpress™)
with rabbits [OLAW&AAALAC-accredited facility] |
Phase III
Purification & QC |
Two-step/three-step purification |
Two-step purification |
TAT |
Starting from 7 weeks |
* GenScript is also experienced in RNA, VLP, (Neo)peptide, recombinant protein, small molecule, cell and other target types.
Mouse anti-ID monoclonal antibody package
Image Credit: GenScript
Customized Mouse Anti-ID Monoclonal Antibody Package. Source: GenScript
|
Target
Type |
Full-length IgG |
scFv/VHH/RNA/VLP/(Neo)peptide/recombinant protein/small molecule/Cell etc. |
ADC/Bi-specific Ab |
Phase I Immunogen Preparation |
F(ab’) 2 preparation |
KLH conjugation |
Fab preparation/KLH
conjugation |
Phase II Animal Immunization |
Conventional immunization protocol
Express immunization protocol (MonoExpress™) Preferred |
Phase III Cell Fusion and Screening |
Cell fusion & one round of subcloning
Deliver supernatants of 20 clones, 2 ml/clone
Confirm the type of anti-ID mAb |
Phase IV
Antibody
Production |
Option 1
Sequencing &
rAb production Preferred |
5 clones sequencing by NGS
Guaranteed 0.1 mg recombinant production and ELISA validation |
Option 2
Clone delivery &
Ab hybridoma production |
Subcloning for up to 5 clones
Clone storage (Optional)
Ab hybridoma production and purification |
TAT |
Starting from 13 weeks |