A novel cationic nanotechnology called jetOPTIMUS® was created to increase the effectiveness of DNA transfection in adherent cells, including difficult-to-transfect cells. Transfection with jetOPTIMUS® requires a minimum amount of DNA and reagent volume to function in pertinent physiological settings while maintaining cell viability and morphology.
Available sizes
- jetOPTIMUS® 0.1 mL
- jetOPTIMUS® 0.75 mL
- jetOPTIMUS® 1.5 mL
- jetOPTIMUS® buffer 60 mL
Capabilities
Superior gene expression in easy-to-hard-to-transfect cells
When employed as in vitro cell culture models, jetOPTIMUS® increases the efficacy of DNA transfection in both easy- and difficult-to-transfect cells. jetOPTIMUS® outperformed its key competitors regarding gene expression and transfection efficiency when tested on various primary cells and cancer cell lines.
Images Credit: Sartorius Corporation
High gene expression using a low DNA amount
Compared to its primary rivals, jetOPTIMUS® has been created to improve transfection efficiency into a variety of cell types while requiring less reagent and minimizing the amount of DNA.
Images Credit: Sartorius Corporation
Excellent cell viability and morphology
When high transfection efficiencies are achieved, they negatively impact the cell cycle, metabolism, and signaling pathways, frequently at the expense of cellular integrity.
Cell integrity is never compromised since the jetOPTIMUS® transfection reagent is gentle on cells, maintaining their vitality and morphology while enabling the production of biologically meaningful data from gene expression analyses.
Images Credit: Sartorius Corporation
Time-saving with optimized ready-to-use protocol
The transfection reagent jetOPTIMUS® comes with its complexation buffer, called jetOPTIMUS® Buffer, and is ready to use. The procedure is designed to be easy to use (all plate sizes), compatible with culture media (antibiotics, serum), and economical (minimum DNA and reagent volume).
Images Credit: Sartorius Corporation
Overview
Even in cells that are difficult to transfect, jetOPTIMUS®, a potent transfection reagent, increases cellular uptake and endosomal escape of DNA in adherent cells, leading to increased transfection efficiency.
When jetOPTIMUS® was tested on various primary cells and cancer cell lines, it demonstrated its superiority by achieving better gene expression and transfection efficiencies with negligible effects on cell viability and morphology.
jetOPTIMUS® is a very effective DNA delivery system for a variety of applications, including small-scale virus manufacturing, CRISPR genome editing utilizing DNA, and temporary and stable gene expression.
Key benefits
- Highly efficient: Maximum expression of genes in cells that are easy and difficult-to-transfect
- Cost-effective: Minimum amount of DNA and reagent volume is needed.
- Biologically relevant: Outstanding morphology and vitality of cells
- Time-saving: Transfection using a ready-to-use, optimized procedure
Specifications
Source: Sartorius Corporation
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| Applications |
Application |
- Transient and stable gene expression from plasmid DNA transfection
- CRISPR Genome editing using DNA approach
- Small-scale virus production
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| Cell Type |
- Adherent cells and hard-to-transfect cells
- Primary cells (epithelial, hepatocyte, endothelial, fibroblast, etc.)
- Stem cells (embryonic, mesenchymal, induced pluripotent)
- Cancer cell lines
- Neurons after fibroblast
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| Biological Information |
Molecule delivered |
Plasmid DNA |
| Number of transfections |
1.5 ml of jetOPTIMUS® transfection reagent is sufficient to perform 3,000 transfections in 24-well plates or 750 transfections in 6-well plates following the standard protocol |
| General Specifications |
Amount of Reagent |
jetOPTIMUS® 1.5 mL |
| Reagents and Kits Type |
jetOPTIMUS® - DNA transfection |
| Product Information |
Provided With |
jetOPTIMUS® Buffer |
| Storage Conditions |
Storage Conditions |
5 °C ± 3 °C |