The use of PULSin® for protein and antibody delivery is a powerful strategy for functional research. By regulating the expression level and the expression time course, PULSin® allows users to study a protein's function and target intracellular proteins with antibodies in living cells without needing a fixation step.
Available size
Capabilities
PULSin® enables the delivery of protein irrespective of its size
Up to 98% of cells can have R-phycoerythrin, a fluorescent protein with a molecular weight of 240 kD, delivered to their cytoplasm by PULSin®. Due to its large size, the protein cannot enter the nucleus and remains evenly distributed in the cytoplasm.
Image Credit: Sartorius Corporation
PULSin® efficiently delivers antibodies in live cells
In mammalian cells, antibodies are effectively transported and can identify their target protein within the cytoplasm. FITC-labeled anti-alpha-tubulin was delivered to the cytoplasm of a significant portion of HeLa cells (85%), thanks to PULSin®.
Similarly, 98% of live HeLa cells received anti-giantin Alexa Fluor® 488 in their cytoplasm, labeling the Golgi apparatus.
Image Credit: Sartorius Corporation
Simple protocol for successful protein delivery
Compared to other methods that use chemical conjugation or viral transduction, PULSin® will save time and effort. A fluorescent control protein (R-phycoerythrin) and a dilution buffer are included with the ready-to-use PULSin® reagent.
The process is quick: just mix the reagent with the protein, let it sit, and then add it to the cells. Analysis of the cells can begin four hours after delivery.
Image Credit: Sartorius Corporation
Easy to use alternative to chemical conjugation and viral transduction
Compared to two other protein delivery reagents, PULSin® exhibits a higher efficiency of protein delivery. PULSin® delivers a greater amount of protein per cell as measured for FITC-alpha-tubulin antibody and R-phycoerythrin protein.
Image Credit: Sartorius Corporation
Overview
PULSin® comprises a proprietary cationic amphiphile molecule that forms non-covalent complexes with proteins and antibodies. These complexes are taken up by anionic cell-adhesion receptors and released into the cytoplasm, where they disassemble. This non-toxic process effectively delivers functional proteins.
Key benefits
- Unique: One reagent for delivering peptides, proteins, and antibodies into mammalian cells
- Optimized: Difficult-to-transfect cells and effective delivery in live primary cells
- Innovative: A simple substitute for viral transduction and chemical conjugation
Specifications
Source: Sartorius Corporation
| . |
. |
. |
| Applications |
Cell Type |
Adherent & suspension cells grown in presence of serum |
| Biological Information |
Hepes dilution buffer |
20 mL |
| Molecule delivered |
Protein, Antibody, Peptide |
| Number of transfections |
0.4 ml of PULSin® delivery reagent is sufficient to perform 25 delivery experiments in 6-well plates and 100 experiments in 24-well plates |
| Positive control (R-phycoerythrin) |
20 µg |
| General Specifications |
Amount of Reagent |
PULSin® 0.4 mL |
| Reagents and Kits Type |
PULSin® - Protein delivery |
| Product Information |
Provided With |
Hepes Buffer (20mM) for protein dilution, R-Phycoerythrin used as a positive control |
| Storage Conditions |
Storage Conditions |
5 °C ± 3 °C |