INTEGRA Biosciences created the MAG and HEATMAG modules with scientists in mind. These state-of-the-art methods simplify the purification of magnetic beads in various proteomics and molecular processes. The strong magnets guarantee quick bead collection with little loss or carryover, and their height may be adjusted to match volume needs for consistently good results.
However, the HEATMAG module goes one step further by fusing heating with magnetic separation, creating the ideal environment for extracting nucleic acids. INTEGRA stands out from the competition thanks to its genuinely original solution.
The MAG and HEATMAG modules provide unprecedented versatility. Depending on the needs, they can be seamlessly linked with pipetting platforms for semi- or completely automated workflows or used as standalone units for manual processing. Combining them with the VIAFLO 96, MINI 96, or VIAFLO 384 pipetting platforms allows users to process 96 or 384 well plates or 24 tubes at the same time, considerably increasing productivity.
The MAG and HEATMAG modules work with the ASSIST PLUS pipetting robot to deliver comprehensive walk-away magnetic bead purification without using expensive grippers or robotic arms. This combination expands the options for the laboratory, making it easier than ever to get high-quality findings.
In conclusion, the main advantages of the MAG and HEATMAG modules are:
- Magnet heights can be adjusted to capture beads based on volume
- Outstanding magnet strength that guarantees maximum yields and less bead carryover
- For lysis or elution, HEATMAG provides a special fusion of heating and magnetic interaction
- Simple compatibility with the pipetting platforms VIAFLO 96, MINI 96, or VIAFLO 384
- Complete automation in conjunction with the pipetting robot ASSIST PLUS
How it works
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MAG modules use hands-free vertical magnet movement to enable automatic bead collecting.
Overview
- Strong magnet ensures minimum bead loss. The MAG modules allow for the exchange of the magnetic array
- Bead collection is automated by moving the magnet vertically
- Different labware formats, such as tubes or plates, can be processed
- The ASSIST PLUS pipetting robot allows users to fully automate magnetic bead purification procedures
Fast bead capture
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High yields are achieved through hands-free vertical movement of a powerful magnetic array and an adjustable bead collection height. This allows for automated bead collection and excellent magnetic bead capture in small and large volumes.
Users can select from various preset heights when used manually, or the ASSIST PLUS pipetting robot provides full workflow automation.
Heating and magnetic separation in one
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HEATMAG combines magnets and heating in one device, allowing for processes at temperatures up to 65 °C. This special property is vital for operations like lysis and elution.
Versatility
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The modules are compatible with various labware types, making them useful for various applications.
Boost productivity for magnetic bead applications
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The integration of MAG modules with the ASSIST PLUS pipetting robot enables completely automated, walk-away sample processing, which improves workflow efficiency and productivity in magnetic bead purification methods.
When combined with the MINI 96, VIAFLO 96, or VIAFLO 384, the modules can handle 96 or 384 wells or 24 tubes simultaneously, allowing for high-throughput magnetic bead clean-up.
Experience intuitive user interaction
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Explore seamless automation with the ASSIST PLUS pipetting robot and VIALAB pipetting software, allowing easy control of the MAG modules. To utilize the modules in conjunction with the MINI 96, VIAFLO 96, or VIAFLO 384, or on their own, connect them to a computer via USB for easy customization or install the connectivity Module to enable Bluetooth connectivity with the MAG Control App on mobile devices.
Sustainability through longevity and plastic reduction
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The longer a device can be utilized, the better for the environment. INTEGRA designs its products to be low-wear and easy to service. They provide disposable, plastic-free options for pipette tips and reservoirs.
See it work
MAG and HEATMAG - Modules for magnetic bead separation
Video Credit: INTEGRA Biosciences
Uses and applications
Overview
PCR clean-up
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PCR product purification using magnetic beads for efficient cleanup
To prevent unincorporated primers or dNTPs from interfering with subsequent reactions, reaction mixes must be cleaned up following PCR procedures. The 96 or 384-well PCR plate adapter for the MAG module allows for hands-free PCR cleanups for up to 384 concurrent reactions per run.
NGS library clean-up
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Single-size and double-size selection in NGS library preparation
When preparing NGS libraries, magnetic beads effectively separate DNA fragments according to their size, essential for both single- and double-size selection. When paired with the accuracy of INTEGRA’s pipetting platforms, magnetic bead purifications using the MAG modules offer great yields with little bead loss.
Nucleic acid extraction
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Magnetic beads simplify nucleic acid extraction, isolating DNA and RNA effectively
Selective binding of DNA or RNA from complicated biological materials is necessary for nucleic acid isolation. Magnetic beads provide scalability in nucleic acid extraction for both sample counts and quantities. To achieve appropriate heights for collection during binding/washing (volumes >500 µl) or elution steps (volumes <200 µl), the bead collection height can be adjusted in between the steps.
Some extraction techniques, such as lysis and elution, require increased temperatures; the HEATMAG module’s heating element, combined with the 1.5 ml tube adapter, is perfect for nucleic acid extractions.
Protein enrichment
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Concentration of specific proteins in a biological sample using magnetic beads
Magnetic beads’ surfaces can also be functionalized with specific ligands, such as antibodies for immunoprecipitation, for application in protein analysis. These ligands are chosen based on their high affinity for the target proteins of interest. Combining MAG modules with INTEGRA’s pipetting platforms reduces the number of repetitive manual pipetting procedures, allowing for more hands-free time.
Applications
- Dependable time-to-result with magnetic beads in a high throughput PCR purification procedure
- An effective process for PCR purification
- Automated DNA size selection for versatile NGS workflow integration
- Proven NGS size selection techniques for the ASSIST PLUS pipetting robot
Tech info
Here is a brief synopsis of the product details.
Source: INTEGRA Biosciences
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Dimensions
(W x D x H) |
MAG: 133 mm x 88.5 mm x 70 mm (5.23 x 3.48 x 2.75 '') — HEATMAG: 133 mm x 88.5 mm x 73 mm (5.23 x 3.48 x 2.87 '') |
| Weight |
MAG: 1.0 kg (2.2 lbs) — HEATMAG: 1.1 kg (2.4 lbs) |
| Operating Conditions |
Temperature: 5 to 40 °C — Humidity: <80 % |
| Power Requirements |
100-240 VAC, 50/60 Hz, device input: 24 V, 120 W |
Image Credit: INTEGRA Biosciences
Image Credit: INTEGRA Biosciences
Image Credit: INTEGRA Biosciences
Image Credit: INTEGRA Biosciences
Image Credit: INTEGRA Biosciences
Image Credit: INTEGRA Biosciences
Image Credit: INTEGRA Biosciences
Image Credit: INTEGRA Biosciences
Image Credit: INTEGRA Biosciences
Image Credit: INTEGRA Biosciences
Image Credit: INTEGRA Biosciences
Image Credit: INTEGRA Biosciences