Three-dimensional epithelial lip area model developed for cosmetics research

KOSÉ Corporation (Headquarters: Chuo-ku, Tokyo; President: Kazutoshi Kobayashi) has developed in collaborative research with Professor Kenji Izumi and his colleagues at Niigata University Graduate School of Medical and Dental Sciences (Faculty of Dentistry) a three-dimensional epithelial model that reproduces the human lip area from the oral mucosa to the lips and surrounding skin, using cell culture.

The lips are one of the most important elements that determine the impressions of the face, and they are also an area where many people suffer from problems, such as chapped lips in winter. The lip area is the transition area between the facial skin and the oral mucosa, and has a unique structure and properties that differ from both of them, such as the stratum corneum being thinner and more delicate than in skin. However, until now, there has been no evaluation system using lip area models, and it has been necessary to use skin and oral mucosa in vitro models to evaluate the usefulness and safety of new ingredients. Therefore, the development of lip area models that reproduce its unique structure, and their use for functional analysis of the lip area and evaluation of formulations and ingredients, is a useful tool to promote better formulation development, says Prof. Izumi.

The research group developed a lip area model with the aim of enabling it to be used in cosmetics research. They used only cells derived from the epidermis and oral mucosal epithelium to develop a three-dimensional model with characteristics similar to those of the human lip area.

The immunohistochemical findings demonstrated that the lip area model developed in this research has a similar structure and differentiation mode to that of actual human lip area tissue. Prof. Izumi describes, it is expected to be used for evaluation of the effects of cosmetic ingredients on the lip area and other purposes.

The lip area model developed in this research will be used for basic research on the lips, which has only been possible until now with alternative methods using skin and oral mucosa, and for future development of lip care products and ingredients. Prof. Izumi states with confidence that we will continue our research and development to enable us to provide evidence-based solutions to customers' lip area problems, such as clarifying the mechanisms of chapped lips and developing useful ingredients.

The results of this research have been published online in the scientific journal Histochemistry and Cell Biology in June 2023.