Scientists develop breakthrough approach to create artificial allosteric sites in protein complexes

Reviewed by Megan Craig, M.Sc.Jul 6 2023

According to a recently published research paper by a team of scientists, a groundbreaking approach has been developed to create artificial allosteric sites (where by binding an effector molecule, activity at the distal active site is regulated) in protein complexes. This breakthrough research holds significant promise for a wide range of applications in industrial, biological, medical, and agricultural fields.

The team's work is published in Nature Chemistry on 06 July 2023 at 16:00 (London time)

Protein complexes, such as hemoglobin and molecular motors, exert concerted functions through cooperative work between the subunits (constituent proteins in the protein complex). This orchestration is enabled by the allosteric mechanism. Allosteric effect, regulation of function at an active site in a subunit by the binding of an effector molecule to an allosteric site in another subunit, was originally proposed in the 1960s and since then it has remained one of the most important topics in the biochemistry field. The research team developed a strategy for designing artificial allosteric sites into protein complexes to regulate a concerted function of a protein complex. "The creation of artificial allosteric sites into protein complexes has the potential to reveal fundamental principles for allostery and serve as tools for synthetic biology," said Nobuyasu Koga, a professor at the Osaka University.

The research team hypothesized that allosteric sites in protein complexes can be created by restoring lost functions of the pseudo-active sites which are predicted to have been lost during evolution. Various protein complexes include subunits that have pseudo-active sites. It has been reported that pseudo-active sites have an allosteric connection with active sites in other subunits. For example, a pseudo-active site in a subunit, which has lost ATPase activity but still exhibits ATP-binding ability, activates another subunit's active site upon binding to ATP. (At the cellular level, ATP is the source of energy. ATPase describes the enzyme's ability to decompose ATP.) Such studies support the idea that distinct allosteric sites can be created into protein complexes by engineering pseudo-active sites.

The research team used computational design to restore the lost ATP-binding ability of the pseudo-active site in the B-subunit of a rotary molecular motor, V₁-ATPase. First, binding ability of the designed site was experimentally revealed by X-ray crystallography. "The X-ray structure indicated the binding site is successfully designed and integrated into the natural protein to have a function. I was amazed at the utility with high performance of protein design technology." said Mikio Tanabe, an associate professor at the Institute of Materials Structure Science. Next, their single-molecule experiments with X-ray crystallography analyses revealed that the binding of ATP to the designed allosteric site boosts this V₁'s activity compared to the wildtype, and the rotation rate can be tuned by modulating the ATP's binding affinity. The team created cooperativity in the rotary motor. Moreover, the team's designed allostery accelerated, not decelerated, the rotation, compared to the wildtype.

To our best knowledge, acceleration of a rotary molecular motor by protein engineering is a first time achievement. This is an exciting result in the field."

Ryota Iino, Professor at the National Institutes of Natural Sciences

Pseudo-active sites are widespread in nature, and their approach shows promise as a means of programming allosteric control over concerted functions of protein complexes. In addition, the protein design method would enable not only to restore the lost function but also to design binding sites for other ligands. "Our strategy enables us to create allosteric sites into various kinds of protein complexes in principle. Our next step is to create allosteric control for a variety of protein complexes by our strategy. Moreover, we will try to design novel functions into pseudo-active sites. One of our goals is to artificially control concerted functions of any protein complexes and to uncover the general mechanism of allostery. We hope allosteric control over concerted functions of protein complexes will open up new avenues in industrial applications of enzymes or biological, medical and agricultural fields," said Takahiro Kosugi an assistant professor at the National Institutes of Natural Sciences.

The research team includes Takahiro Kosugi from the Institute for Molecular Science (IMS) National Institutes of Natural Sciences (NINS), the Exploratory Research Center on Life and Living Systems (ExCELLS) NINS, SOKENDAI (The Graduate University for Advanced Studies), and PRESTO Japan Science and Technology Agency; Tatsuya Iida and Ryota Iino from IMS NINS and SOKENDAI; Mikio Tanabe from the Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK); and Nobuyasu Koga from IMS NINS, ExCELLS NINS, SOKENDAI, and the Osaka University.

This research is funded by a Grant-in-Aid for Scientific Research on Innovative Areas "Molecular Engine", Japan Science and Technology Agency "Precursory Research for Embryonic Science and Technology", and the National Institute for Natural Sciences, Okazaki Institute for Integrative Bioscience. Diffraction experiments for X-ray crystallography were performed at the KEK Photon Factory BL-1A partly supported by AMED-BINDS. The computation was performed using Research Center for Computational Science, Okazaki, Japan.