New instruction manual could help scientists find hard-to-detect B cells

Researchers with McMaster University have created the instruction manual that will help scientists across the globe find hard-to-detect B cells.

Led by PhD student Alyssa Phelps and Department of Medicine Assistant Professor Josh Koenig, researchers wanted to chart a path to finding these cells as part of their work in understanding food allergies. Their work was published in the journal Nature Protocols on Jan. 19, 2024.

B cells are a type of immune cell that makes antibodies. These cells help fight conditions like cancer and infections but can also cause autoimmune diseases and allergies.

One of the big problems with trying to study these B cells, the ones that make these antibodies that have all kinds of different and very important functions, is that they're really, really, rare. It's hard to find them. And so, you have to have very good tools that will help you study these things."

Josh Koenig, Assistant Professor, Department of Medicine, McMaster University

To give an example of just how rare these cells can be, Koenig pointed to a peanut-specific B cell. It makes up less than 0.0001 per cent of immune cells in human blood.

The team adopted a method originally created by Justin Taylor, who now operates the Taylor Lab out of the University of Virginia. Taylor created a method using antigen tetramers to sensitively tag and enrich specific B cells so they can be detected.

Tetramers are made up of four antigen molecules, which in this case, can be customized by scientists. The customization is vast and can cover everything from peanuts to COVID-19-specific B cells.

"After using the technology for a few years in several of our studies and making multiple different allergen tetramers and antigen tetramers for other people, we decided to write up a protocol paper to help other people study these incredibly important B cells," says Phelps.

In addition to better understanding how allergies work in humans, tetramers can be used to study the efficacy of vaccines. This is something that was done by Koenig and his team in assisting McMaster researchers Matthew Miller, Brian Lichty, and Zhou Xing in determining whether their vaccine candidate activated protective COVID-specific B cells.

"With these protocols now published, more researchers around the globe will be able to make these types of tools to help to push their science ahead," Taylor says.

The study was supported in part by a $10 million donation by the Schroeder Foundation to McMaster University. Funding was also provided from ALK Abello A/S, a pharmaceutical company based in Denmark.