Moffitt study identifies predictive biomarkers for immunotherapy in merkel cell carcinoma

Merkel cell carcinoma is a rare but highly aggressive form of skin cancer known for its rapid growth and tendency to metastasize. Despite the promise of immune checkpoint blockade therapy, which can boost the body's immune response against cancer cells, nearly half of patients do not respond to this treatment. A new study published today in Cancer Discovery is providing insights into why some Merkel cell carcinoma patients respond to this type of immunotherapy while others do not.

In collaboration with scientists at Robert H. Lurie Comprehensive Cancer Center of Northwestern University, Moffitt Cancer Center researchers conducted the largest and most comprehensive study to date on Merkel cell carcinoma. They analyzed samples from 116 patients using advanced multimodal techniques -; bulk and single cell RNA sequencing, spatial transcriptomics and multiplexed immunofluorescence -; to gain insights into the immune response and tumor characteristics. Their findings reveal that specific immune cells, particularly tissue resident CD8 T cells and γδ T cells, play a crucial role in the body's response to immune checkpoint blockade therapy.

The research team, co-led by Kenneth Tsai, M.D., Ph.D., vice chair of Pathology Research at Moffitt, and Jaehyuk Choi, M.D., Ph.D., at Northwestern University, discovered that those who respond to immune checkpoint blockade therapy have higher levels of preexisting tissue-resident CD8 T cells or Vδ1 γδ T cells within their tumors. These cells exhibit unique transcriptional programs and clonal expansion reflective of antigen specificity, meaning they can effectively recognize and attack cancer cells. In contrast, tumors from those who do not respond to immunotherapy showed increased proliferation and markers associated with neuronal stem cells, as well as the inflammatory molecule IL-1.

Using spatial transcriptomics, the researchers demonstrated that these beneficial T cells are often found in close proximity to other immune cells like B cells and dendritic cells, which help enhance their activity by supplying necessary chemokines and costimulation. This close cellular interaction within the tumor microenvironment is a key factor in the effectiveness of the immune response.

"Our findings not only highlight the potential to use specific genes and immune cells as biomarkers for predicting patient response to immune checkpoint blockade therapy, but also suggest several approaches for abrogating resistance and enhancing efficacy," Tsai said. "Importantly, patients with tumors already containing the right mix of immune cells before treatment were more likely to respond, suggesting that increasing their numbers with the correct localization could enhance treatment outcomes."

The Cutaneous Oncology Department and the Donald A. Adam Melanoma and Skin Cancer Center of Excellence at Moffitt provided critical support and represent leading clinical and research expertise in Merkel cell carcinoma.

This research was supported by the National Institutes of Health (DP2 OD024475-0, R35 GM144083P30 CA076292, P30 AR075049, P30 CA060553, T32 CA009560 and F30CA278298), Barry S. Greene Fund, V Foundation for Cancer Research (T2021-019) and Leukemia and Lymphoma Society (1377-21).