Temperature changes influence meiotic crossovers and chromosome organization in yeast

Science China PressAug 23 2024

This study is led by Prof. Shunxin Wang (State Key Laboratory of Reproductive Medicine and Offspring Health, Center for Reproductive Medicine, Institute of Women, Children and Reproductive Health, Shandong University) and Prof. Liangran Zhang (Advanced Medical Research Institute, Shandong University). In their study of meiosis in budding yeast, the research team found that yeast senses temperature changes by increasing the level of DNA negative supercoils to increase crossovers and modulate chromosome organization during meiosis.

Meiosis is a specialized cell division producing gametes with the half chromosome complement of their progenitor cells. Meiotic crossovers between homologous (maternal and paternal) chromosomes, which result in the reciprocal exchange of chromosome fragments, play two important roles: physically holding the homologous chromosomes together to ensure their proper segregation, and promoting the genetic diversity of their progeny. The formation of crossovers is regulated by the architecture of meiotic chromosomes, each of which is organized as a linear array of loops anchored at their bases to a proteinaceous axis.

The researchers studied yeast meiosis, and found that changes in temperature (either decreased or increased) resulted in shorter meiotic chromosome axes and more crossovers. The research teams further found that temperature changes coordinately enhanced the hyperabundant distribution of axis proteins (such as Red1 and Hop1) on chromosomes and the number of putative crossover marker Zip3 foci. Importantly, temperature-induced changes in the distribution of axis proteins and Zip3 foci depend on changes in DNA negative supercoils, which have been shown to regulate the number of crossovers. In addition, temperature changes regulate the abundance of axis-associated proteins and thus axis length, independently of changes in DNA negative supercoils. These results suggest that yeast meiosis senses temperature changes by increasing the level of DNA negative supercoils to increase the number of crossovers and modulate chromosome organization.

These findings provide a new perspective on understanding the effect and mechanism of temperature on meiotic crossovers and chromosome organization, with important implications for evolution and breeding.