The depth of structural information provided by NMR spectroscopy has made it useful for conventional small-molecule medicinal and analytical chemistry applications. However, its ability to effectively but precisely characterize peptides and oligonucleotides (TIDES) is somewhat less well-known.
This webinar will explore its effectiveness with examples spanning TIDES structure-based drug design,1,2 similarity evaluation,3 characterizations,4 secondary and tertiary structure, quantification, and phosphorothioate chirality.5,6
Speakers
Codina holds a degree in Chemistry and a Ph.D. in Protein NMR from the University of Barcelona, Spain. She did her postdoc in protein NMR at the MRC Laboratory of Molecular Biology in Cambridge, UK.
She then spent seven years in Pfizer's Analytical R&D department, where she learned how to elucidate low-level impurity structures, monitor reactions, use qNMR, and provide regulatory documentation.
Codina also received the Pfizer Worldwide Achievement Award for using NMR-based reaction monitoring in an open-access environment.
She later joined Bruker in 2011 and has held many roles since then, including Material Characterization Laboratory Manager, Product Portfolio Manager, and, for several years, driving the Biopharmaceutical business at Bruker BioSpin. More recently, Codina joined SciY as Senior Director of Strategy and Business Development.
References
- Haxholm, G.W., Petersen, B.O. and Malmstrøm, J. (2019). Higher-Order Structure Characterization of Pharmaceutical Proteins by 2D Nuclear Magnetic Resonance Methyl Fingerprinting. Journal of Pharmaceutical Sciences, 108(9), pp.3029–3035. https://doi.org/10.1016/j.xphs.2019.04.032.
- Gelenter, M.D., et al. (2019). The peptide hormone glucagon forms amyloid fibrils with two coexisting β-strand conformations. Nature Structural & Molecular Biology, 26(7), pp.592–598. https://doi.org/10.1038/s41594-019-0238-6.
- Becette, O.B., Marino, J.P. and Brinson, R.G. (2022). Structural Fingerprinting of Antisense Oligonucleotide Therapeutics by Solution NMR Spectroscopy. Pharmaceutical Research, 40(6), pp.1373–1382. https://doi.org/10.1007/s11095-022-03403-x.
- Food and Drug Administration (2023). Draft guidance on givosiran sodium. Available at: https://www.accessdata.fda.gov/drugsatfda_docs/psg/PSG_212194.pdf.
- Jahns, H., et al. (2021). Chirality matters: stereo-defined phosphorothioate linkages at the termini of small interfering RNAs improve pharmacology in vivo. Nucleic acids research, (online) 50(3), pp.1221–1240. https://doi.org/10.1093/nar/gkab544.
- Genna, V., et al. (2023). Controlled sulfur-based engineering confers mouldability to phosphorothioate antisense oligonucleotides. Nucleic Acids Research, 51(10), pp.4713–4725. https://doi.org/10.1093/nar/gkad309.