Watch this dynamic webinar for valuable insights into characterizing the stability of an engineered adeno-associated virus (AAV) serotype under moderate thermal stress and physiological solution conditions, utilizing light scattering measurements. AAVs are at the forefront of gene therapy vector systems, yet gaps persist in our understanding of their instability and the resulting DNA ejection from AAV capsids due to various thermal, chemical, and mechanical stresses.
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This webinar showcases experiments that explored the effects of thermal perturbations at three temperature setpoints on DNA ejection from a proprietary AAV vector, as well as the determination of absolute molecular weights for both full and empty capsid proteins. Notably, the study found that AAV capsid proteins aggregated immediately following DNA ejection, while full capsids with DNA aggregated more slowly. This finding suggests that DNA may play a crucial role in maintaining capsid structure and preventing protein aggregation.
Understanding the quantitative and qualitative properties of viral vector systems is essential to ensure their maximum efficacy and stability during processing, storage, and administration. Watch the informative webinar today to gain key insights into optimizing the efficacy and storage conditions of adeno-associated virus pharmaceuticals.
Professor Wayne Reed is a co-founder and Scientific Advisor at Yokogawa Fluence Analytics and the inventor behind the technologies that form the foundation of the company’s product portfolio. He is the Murchison-Mallory Chair in Physics and is the Founding Director of PolyRMC at Tulane University.
Prof. Reed earned his Ph.D. in Physics from Clarkson University, an M.S. from the University of Washington, and a B.S. from Rensselaer Polytechnic Institute. Renowned for his expertise in data analysis and characterization methods for synthetic and natural polymers, he has authored over 150 publications and is named as an inventor on more than 30 issued patents and patent applications.
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