This Quick Protocol provides instructions for use of the Maxwell® RSC PureFood Pathogen Kit with the Maxwell® RSC Instrument to purify pathogen DNA from food samples. For detailed instructions, including information on instrument setup and troubleshooting, please refer to the Maxwell® RSC PureFood Pathogen Kit Technical Manual #TM528, available at: www.promega.com/protocols/
Image credit: Promega
Materials to be Supplied by the User
- microcentrifuge tubes, 1.5 ml or 2.0 ml
- sterile, aerosol-resistant pipette tips
- ThermoMixer® heat block
- Stomacher® lab paddle or vortexer
- Stomacher® bags
- 25 g of food sample
Download the full article to see the protocol for bacterial lysis from food matrix and automated DNA purification
Download the Full Article
About Promega
With a portfolio of more than 3,000 products covering the fields of genomics, protein analysis and expression, cellular analysis, drug discovery and genetic identity, Promega is a global leader in providing innovative solutions and technical support to life scientists in academic, industrial and government settings.
Promega products are used by life scientists who are asking fundamental questions about biological processes as well as by scientists who are applying scientific knowledge to diagnose and treat diseases, discover new therapeutics, and use genetics and DNA testing for human identification.
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