Pipetting low and high-density liquids

The handling of difficult liquids can be quite a challenge in a daily lab routine, whether they are viscous, fluorescent, or light-sensitive substances or reagents that need extensive strict aseptic conditions. The following list of easy-to-follow steps will help guide users through the pipetting and handling of said challenging liquids.

Pipetting low vs. high density liquids

Pipetting low and high-density liquids

Image Credit: AHN Biotechnologie GmbH

Tips with an exceptionally small orifice are ideal for use with low-density liquids, like PCR reagents. The extra narrow tip orifice, in combination with the extended length of the tip, means that even a less skilled user can achieve consistency in their pipetting protocol, significantly improving the reproducibility of their pipetting results.

Due to their slightly wider tip orifice, the standard 10 µl tips tend to be the best option for liquids with densities higher than water.

Sample care is needed to achieve high precision

Pipetting low and high-density liquids

Standard Tip (left) and ExpellPlus™ low retention (right). Image Credit: AHN Biotechnologie GmbH

The low retention tips are the best choice if top accuracy and reproducibility are required or when working with delicate or expensive substances that need to be handled with extra care.

The unique mixture of the plastic polymer provides an ultra smooth surface inside the tip.

Capp ExpellPlus® Low Retention Tips

Capp ExpellPlus® Low Retention Tips. Video Credit: AHN Biotechnologie GmbH

Capp Expell® Reload System Tips

Capp Expell® Reload System Tips. Video Credit: AHN Biotechnologie GmbH

AHN Sustainability Approach Video

AHN Sustainability Approach. Video Credit: AHN Biotechnologie GmbH

Samples requiring the strictest aseptic conditions

The individually wrapped tips for handling samples that need to be handled under the strictest aseptic conditions. The single-wrap packaging maximizes the exceptional performance of the sterile tips by guaranteeing sterility for every pipetting cycle.

Handling fluorescent or light-sensitive substances

Some users wrap their light-sensitive samples in aluminum foil before processing them to shield them from the impact of light. The black microcentrifuge tubes can be a fantastic solution when working with photosensitive, fluorescent, or ethidium bromide probes.

The black microcentrifuge tubes, in contrast to amber-tinted tubes, are entirely opaque and can be used with even the most light-sensitive samples.

About AHN Biotechnologie GmbH

AHN Biotechnologie GmbH, based in Nordhausen, Germany, is a manufacturer of plastic injection molded products, life science products and laboratory consumables for worldwide laboratory use.

With over 20 years’ manufacturing experience in serving German and global leaders in the Laboratory Automation Industry, we have been acting as a global OEM manufacturer of consumables for many outstanding life science brands and distributors. Our state-of-the-art manufacturing facility meets and exceeds the rigorous standards and quality requirements of the best-known brands we supply.

At the same time, we are strengthening our three associated brands: CAPP, AHN and Maxxline by producing and supplying a comprehensive range of consumables, liquid handling devices and laboratory equipment. Our unique combination of flexibility, innovation and high-quality production automation ensures that our laboratory products meet the needs of customers worldwide and meet their high-quality requirements.

With our expertise in manufacturing laboratory consumables and the state-of-the-art electric injection molding technology that offers faster production turnaround time and sustainable quality products, we achieve the best value for money that meets the strict standards of the modern laboratory industry.

Sustainability is built into every part of our production flow and is reflected in all our products, such as the plastic-saving pipette tip refill systems, the cardboard racks for pipette tips or cryovials.


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Last updated: Jan 13, 2023 at 8:44 AM

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