How to achieve arsenic speciation

Organic arsenic compounds added to chicken and poultry feedstocks represent significant environmental and ecological risks.

National and international health bodies, including the United States Environmental Protective Agency and the US Food and Drug Administration, have recently imposed stringent concentration limits for arsenic species in drinking water and food.

The variety of sample matrices used complicates arsenic species analysis. To address these issues, an HPLC-ICPMS approach has been devised.

Arsenic compounds are resolved using ion exchange chromatography on a Hamilton PRP-X100 column, a 55 % cross-linked polystyrene-divinylbenzene copolymer functionalized with a quaternary ammonium anion exchanger group. The detection method is inductively coupled plasma-mass spectrometry.

Source: Hamilton Company

Column Information
Column Hamilton PRP-X100, 5 μm, 4.6 x 250 mm
Part Number 79181
FlowRate 1.0 mL/min
Mobile Phase 2 mM (NH4)2CO3 for 0–3 min
text 40 mM (NH4)2CO3 for 3–14 min
text 2 mM (NH4)2CO3 for 13–17 min
Injection Volume 50 μL, 100 μg/L of each standard
Detection ICP-MS

 

Borrowed (with permission) from P. Alava et al. Biomed. Chromatogr. 2012; 26: 524–533

Source: Hamilton Company

Ordering Information
Part Number Product Name Particle Size Dimensions Material
79852 PRP-X100 5 μm 2.1 x 150 mm PEEK
79190 PRP-X100 5 μm 2.1 x 250 mm PEEK
79810 PRP-X100 5 μm 4.1 x 50 mm Stainless Steel
79538 PRP-X100 5 μm 4.1 x 100 mm Stainless Steel
79812 PRP-X100 5 μm 4.1 x 150 mm Stainless Steel
79174 PRP-X100 5 μm 4.6 x 150 mm Stainless Steel
79181 PRP-X100 5 μm 4.6 x 250 mm PEEK
79421 PRP-X100 10 μm 2.1 x 150 mm PEEK
79346 PRP-X100 10 μm 2.1 x 250 mm Stainless Steel
79365 PRP-X100 10 μm 4.1 x 50 mm Stainless Steel
79439 PRP-X100 10 μm 4.1 x 100 mm Stainless Steel
79434 PRP-X100 10 μm 4.1 x 150 mm Stainless Steel
79433 PRP-X100 10 μm 4.1 x 250 mm Stainless Steel
79354 PRP-X100 10 μm 4.6 x 150 mm PEEK
79455 PRP-X100 10 μm 4.6 x 250 mm PEEK
79353 PRP-X100 12–20 μm 21.2 x 250 mm Stainless Steel

 

About Hamilton

Hamilton Company is a global manufacturer and provider of top-tier analytical components, medical instrumentation, temperature control systems, laboratory robotics, and automated liquid handling equipment.

Hamilton Company has been developing and manufacturing pressure-stable, polymeric polystyrenedivinylbenzene (PS-DVB) HPLC columns for over 35 years. These columns are employed in most of the world's top chromatography laboratories.

Hamilton polymeric columns are the chromatographer's choice for difficult separations. They offer a wide range of particle and pore sizes, pH stability from 1 to 14, temperature resistance above 100 °C, and chemistries that match most analyte types.

About Hamilton Company

Hamilton — The Measure of Excellence

Hamilton Company specializes in the development, manufacturing and customization of precision measurement devices, automated liquid handling workstations, and sample management systems. Hamilton's processes are optimized for quality and flexibility. Whether it's a custom needle with a quick delivery time frame, a special length pH sensor, or a comprehensive solution to fully automate your assay workflow, trust that Hamilton products will always meet your needs.

Hamilton Company has been a leading global manufacturer for more than 60 years, with headquarters in Reno, Nevada; Franklin, Massachusetts; Timișoara, Romania; Bonaduz, Switzerland; and subsidiary offices throughout the world.


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Last updated: Oct 2, 2024 at 9:56 AM

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