Data independent acquisition (DIA) has been an important catalyst for transferring mass spectrometry (MS)-based omics from a discovery-focused science to one that more wholly enables longitudinal research, as needed in precision medicine and clinical studies.
The obstacles linked to enhancing throughput in high-resolution MS while optimizing depth, reproducibility, and quantitative precision have led to ZT Scan DIA.
ZT Scan DIA is poised as SCIEX’s subsequent iteration of DIA development after SWATH DIA and, more recently, Zeno SWATH DIA. Since SCIEX launched SWATH DIA in 2011, data-independent acquisition has increasingly become the preferred approach for scientists using liquid chromatography-tandem mass spectrometry (LC-MS/MS).
SWATH DIA is an LC-MS/MS workflow that takes advantage of speed and sensitivity gains that can be achieved via contemporary MS systems. It allows for the more comprehensive identification of large quantities of proteins, peptides, and metabolites than typical approaches to data-dependent acquisition.
With SWATH DIA, high-quality, high-resolution MS/MS data provides identification and accurate and precise quantification.
The Zeno trap1,2, a new accumulation and pulsing device, empowered a new age of sensitivity for accurate mass tools. In Zeno SWATH DIA, once activated, the Zeno trap is utilized to raise MS/MS sensitivity for the required variable windows.3
The Zeno trap increases sensitivity by 4-20 times for Zeno SWATH DIA while preserving other important performance qualities.4
In this article, SCIEX introduces an innovative DIA method, ZT Scan DIA, which merges the depth of DIA approaches with the precision of targeted methods. Similar to Zeno SWATH DIA, ZT Scan DIA makes the identification and quantification of analytes via MS/MS information possible.
Therefore, the fast acquisition of quality MS/MS data over the whole precursor ion space is key to operation. With the added dimension of information from quadrupole scanning, ZT Scan DIA makes it possible for researchers to uncover the complexity of biological samples by increasing depth and certainty throughout quantitative assessments.
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References and further reading
- Chernushevich, I.V., Merenbloom, S.I., Liu, S., Bloomfield, N. A W-geometry ortho-TOF MS with high resolution and up to 100% duty cycle for MS/MS. J. Am. Soc. Mass Spectrom., 28, 2143-2150 (2017).
- Zeno trap: Defining new levels of sensitivity. (2021) SCIEX white paper, RUO-MKT-19-13373-B.
- Wang, Z. et al. High-throughput proteomics of nanogram-scale samples with Zeno SWATH DIA. BioRxiv preprint: https://www.biorxiv.org/content/10.1101/2022.04.14.488299v1.full
- Qualitative flexibility combined with quantitative power: Using the ZenoTOF 7600 system, powered by SCIEX OS software (2021) SCIEX technical note, RUO-MKT-02-13053-B.
About SCIEX
SCIEX mission is to deliver solutions for the precision detection and quantitation of molecules, empowering their customers to protect and advance the wellness and safety of all.
SCIEX has led the field of mass spectrometry for 50 years. From the moment we launched the first ever commercially successful triple quad in 1981, they have developed groundbreaking technologies and solutions that influence life-changing research and outcomes.
Today, as part of the Danaher family of global life science and technology innovators, they continue to pioneer robust solutions in mass spectrometry and capillary electrophoresis. But they don’t just develop products. It is what they do together with their customers that sets them apart. That’s why thousands of life science experts around the world choose SCIEX to get the answers they can trust to better inform critical decisions. Decisions that positively impact lives.
They proudly stand behind our tagline: The Power of Precision.
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