Users can predict more complex, latent, and immune-related hepatotoxicity in vitro with CN Bio’s PhysioMimix® DILI assay kit: Human 24.

Human 24 DILI assays

Image Credit: CN Bio Innovations Ltd

The PhysioMimix DILI assay kit has everything you need to recreate CN Bio's FDA-recognized assay in your lab.

The DILI Assay Kit: Human 24 provides critical insights beyond intrinsic DILI predictions, helping you make more informed decisions ahead of in vivo animal and first-in-human studies. This all-in-one kit, combined with CN Bio's PhysioMimix OOC range of Single- and Multi-organ Systems, enables the detection of complex, latent, and immune-related hepatotoxicity events.

The DILI assay kit offers:

Effortless adoption – Quickly start using the most up-to-date Organ-on-a-chip technology with ease

All-in-one kit – Gain direct access to top-quality validated cells and reagents to reliably replicate CN Bio’s PhysioMimix DILI assay 

Proven solution – Take advantage of validated protocols, developed through years of experience, strengthened through industry collaboration.

Human relevance – Leverage a human-derived model that facilitates testing for novel drug modalities, with human-specific targets and pathways

Comprehensive support – Enjoy instrument and application support for all parts of the assay

Features of the DILI assay kit

Close the knowledge gap between conventional in vitro and first-in-human research with CN Bio’s PhysioMimix DILI assay kit: Human 24.

  • 24-replicate kit: to profile eight conditions in triplicate, including positive and negative controls
  • Complement in vivo research: with human in vitro data signals potential interspecies differences and diminishes reliance on animal model data
  • Unique Insights: from 3D liver tissues, consisting of primary human hepatocytes and Kupffer cells, over 14 days of culture
  • Key metabolic enzyme expression: including CYP enzymes
  • Mechanistic profiling: using the large volumes of recoverable tissue and media for longitudinal and endpoint analysis to characterize DILI pathways
  • Enhanced clinical translatability: by testing biomarkers, such as ALT/AST, albumin, urea, and LDH

What is in the DILI assay kit: Human 24?

Media. Source: CN Bio Innovations Ltd

Media Volume
Liver Basal A Media 150 mL
Liver Basal B Media 500 mL
Thawing Media 50 mL

 

Supplements. Source: CN Bio Innovations Ltd

Supplements Volume
Supplement I 7 x 10 µL
Positive Control 4 x 50 µL
Negative Control 4 x 50 µL

 

PhysioMimix plates. Source: CN Bio Innovations Ltd

Multi-chip plates Number of plates/replicates
Liver-12+ plates 2 plates with 24 replicates

 

Primary human cells. Source: CN Bio Innovations Ltd

Primary human cells Number of cells
Primary Human Hepatocytes 2 vials
Kupffer cells 2 vials

 

Validation report

Browse CN Bio’s sample report that describes the detailed process of validating the primary human hepatocytes and Kupffer cells utilized in the DILI assay kit: Human 24.

The quality control procedures outlined enable you to culture stable, human-relevant liver microtissues that are metabolically active and immune-competent.

Download report

How does CN Bio’s DILI assay kit compare?

Scientists have access to a broad spectrum of preclinical studies and development tools that can be used to assess DILI risks. How does the DILI assay kit, Human 24, compare to more conventional methods?

  Liver monolayer / spheroid Animal model PhysioMimix DILI assay
Human
Human-relevant CYP activity
Mechanistic toxicity
Advanced drug modalities
Throughput
Cost $ $$$ $$

 

 

About CN Bio Innovations Ltd

CN Bio is a leading organ-on-a-chip (OOC) company that offers a portfolio of products and contract research services to optimize the accuracy and efficiency of bringing new medicines to market. With more than a decade of research and development experience, they aim to transform the way human-relevant pre-clinical data is generated through the development of advanced in vitro human organ models.

CN-Bio's PhysioMimix® OOC range of microphysiological systems (MPS) enable researchers to recreate human biology in the lab. This technology bridges the gap between traditional cell culture and human studies, to support the development of safer and more efficacious therapeutics, whilst reducing the dependence on animal model usage.

CN Bio’s portfolio of products (Organ-on-a-chip systems, assay kits, 3D validated cells, consumable plates) and services support researchers that require reliable, data-rich, physiologically relevant, in vitro studies, to uncover novel mechanistic insights into drug or disease mechanism of action.


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