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Micromanipulation and Microinjection Techniques

Microinjection refers to a technique wherein substances are injected into single cells using a very thin needle.

18 Jan 2019

Metabolic Changes in Aging Cells

Aging is caused by the deterioration of various cellular organelles and physiological processes.

18 Jan 2019

How Should Antibodies be Stored?

If stored incorrectly, the survival of the antibody can be dramatically reduced, lasting only a few weeks rather than a few years.

18 Jan 2019

How do Immunoassays Work?

There are various types of immunoassays, and these are described here.

18 Jan 2019

How to Present Flow Cytometry Data in a Publication

This article provides advice on presenting flow cytometry data in an academic publication, including presenting controls and standardizing the data.

17 Jan 2019

Fluorescence Quenching

Fluorescence quenching is a physicochemical process that lowers the intensity of emitted light from fluorescent molecules.

17 Jan 2019

Eosinophil Function

This article provides a brief overview of eosinophils; specialized cells of the immune system involved in anti-parasitic and inflammatory processes.

17 Jan 2019

Electrochemistry Mass Spectrometry (EC-MS) Applications

EC-MS can be used to analyze a wide range of biological reactions, including redox reactions and protein structures.

17 Jan 2019

Applications of Parallel Accumulation and Serial Fragmentation (PASEF)

PASEF traps precursor ions eluted during liquid chromatography in a tunnel, where they are accumulated and ordered depending on their collisional cross-sectional area and charge.

17 Jan 2019

Why are Beta-Actin and GADPH Used as Controls in Western Blotting?

Beta-actin is commonly used as a western blot loading control as is expressed within all eukaryotic cell types and is not affected by cellular treatments.

14 Jan 2019

Transfer-PCR (tPCR) Steps

tPCR involves three steps; PCR amplification, an intermediate step which creates megaprimers, and the introduction of the gene to the destination vector.

14 Jan 2019

The Tau (CDK5) Signaling Pathway

In Alzheimer’s disease, CDK5 phosphorylates Tau, leading to the accumulation of tau within the axons of nerve cells and eventually, apoptosis.

14 Jan 2019

Affinity Chromatography vs. Protein A/G Purification

Affinity chromatography and protein A/G purification are antibody purification methods to remove ligands from extracts. They have different applications.

14 Jan 2019

What are Bispecific Monoclonal Antibodies?

Bispecific antibodies are comprised of two monoclonal antibodies held together by a flexible peptide linker.

10 Jan 2019

What is the CLARITY Technique?

CLARITY is a method used for the analysis of proteins and nucleic acid location within the brain.

9 Jan 2019

Protein A/G Purification for Antibodies

The protein A/G purification method is used to purify monoclonal IgA and IgG antibodies using a recombinant of Protein A and Protein G.

9 Jan 2019

History of Electrochemistry

Electrochemistry refers to the study of the chemical processes that are responsible for the movement of electrons which, in turn, causes electricity.

9 Jan 2019

Transfer-PCR (tPCR) Applications

In tPCR, target DNA is amplified and integrated into a destination vector in a single tube, without the need to purify the intermediate PCR product.

9 Jan 2019

How are Antibodies Validated?

Antibody validation ensures that antibodies are selective, reproducible, and specific enough for their application.

8 Jan 2019

HEK293 Cells: Applications and Advantages

Human embryonic kidney 293 (HEK293) cells are one of the most common cell lines used for research purposes. This article examines why they are so popular.

8 Jan 2019