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Applications of Thin Layer Chromatography

Thin layer chromatography has wide field of applications that include pharmaceuticals, food, cosmetics and phytochemistry.

18 Sep 2018

Flash Column Chromatography Methodology

Flash column chromatography was developed in 1978 as an alternative method to long column chromatography.

18 Sep 2018

High Performance Liquid Chromatography (HPLC) Applications

High performance liquid chromatography, more commonly known by the acronym HPLC, is a method used to identify substances in a mixture. There are several types of HPLC, such as reversed-phase, denaturing, and immobilized enzyme reactor (IMER) HPLC.

18 Sep 2018

MicroRNA Profiling Methods and Applications

MicroRNAs (miRNAs) are small, short non-coding RNAs that bind to specific messenger RNAs (mRNAs) and regulate gene expression either by degradation or by translational inhibition of the mRNA.

18 Sep 2018

Tissue Nanotransfection Methodology

The application of nanotechnology to the field of medicine and healthcare offers the possibility to produce innovative nanoengineering techniques in the repair and regeneration of tissues and organs.

17 Sep 2018

Analyzing Mitohormesis

When ingested by humans, micro-doses of toxins result in a positive adaptive immune response in cells and organs, whereas higher-doses have detrimental effects. This biphasic response is known as hormesis and comes from the Greek word which translates as ‘to impel’.

17 Sep 2018

What is Methylation-Specific PCR?

DNA methylation is an important epigenetic process which has diverse roles to regulate gene expression, imprinted genes, inactivation of X chromosomes, and the dysregulation of these patterns in diseases.

17 Sep 2018

Thin Layer Chromatography

Thin layer chromatography is used to analyse mixtures: determine the number, identity, and purity of compounds present in a mixture. This technique can also be used to visualize the progress of a reaction.

17 Sep 2018

What is Mitohormesis?

Mitohormesis is a process wherein reactive oxygen species (ROS) produced by mitochondria at a low concentration act as signaling molecules to initiate a cascade of cellular events that ultimately protect the cells from harmful effects.

17 Sep 2018

Chromatin Remodeling: Mechanisms and Importance

Chromatin remodeling is an important mechanism of regulating eukaryotic gene expression, which makes tightly condensed DNA accessible to various regulatory factors, such as transcription factors and components of DNA replication.

13 Sep 2018

Shotgun Metagenomic Sequencing

Diverse microbial communities exist all around Earth, both within our bodies and our external environment.

13 Sep 2018

The Lac Operon in E. Coli

Required for the transport and metabolism of lactose in Escherichia Coli (E. coli).The lactose operon or lac operon consists of three structural genes namely lacZ, lacY, and lacA that encode proteins involved in lactose metabolism as well as several regulatory genes.

12 Sep 2018

What are P-Bodies?

P-bodies are dynamic ribonucleoprotein granules found in the cell cytoplasm. They are primarily composed of translationally repressed mRNAs and proteins associated with mRNA decay machinery.

12 Sep 2018

Single Plane Illumination Microscopy

Single Plane Illumination Microscopy (SPIM) is a non-invasive method of observing an organism and gaining as much biological data from the specimen as possible.

11 Sep 2018

What is Aspergillus niger?

The fungus Aspergillus niger is a type of mould, which can sometimes be attributed to the cause of some cases of pneumonia. It is also the causative agent of ‘black mould’ on the outsides of certain foods, such as apricots, onions, grapes, etc - therefore making Aspergillus niger a food ‘spoilage’ organism.

11 Sep 2018

What is Expansion Microscopy?

Expansion microscopy, put simply, is a method of improving the final image resolution during regular light microscopy, by physically enlarging the organism itself.

10 Sep 2018

16S rRNA Sequencing

For centuries the classification of living organisms into distinct groups and species was done by morphology. While this was sufficient for Darwin and early biologists to study evolution and relatedness of some organisms, this method becomes difficult when characteristics vary.

10 Sep 2018

Lung Organoids: Methodology, Research and Applications

The lungs are comprised of a complex branching system, lined with highly specialized cells. These cells facilitate several crucial functions, such as oxygen exchange, but also immune functions to protect this vulnerable part of the body from infection.

10 Sep 2018

What is the Carbohydrate-Active Enzyme Classification System?

The Carbohydrate-Active enZYme (CAZymes) classification system is a database that contains the knowledge of enzymes that are involved in the formation and break down of complex carbohydrates and glycoconjugates. The database classifies each enzyme based on its role.

6 Sep 2018

Stochastic Optical Reconstruction Microscopy Applications

Stochastic optical reconstruction microscopy (STORM) is a super-resolution microscopy technique with the capability to image in 2D or 3D, in multiple colors, and can even image live cells. This technique has many uses in research.

6 Sep 2018