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Countercurrent chromatography

Countercurrent chromatography (CCC) can be defined as "a technique of chromatographic separation based on the partition of solutes between two different liquid phases that does not get mixed together. The two phases are mobile phase and stationary phase (the support-free liquid)".

4 Dec 2017

Chiral Column Chromatography

Chiral column chromatography makes use of the concept of chirality. Chirality relates to study of the three-dimensional structure of molecules. The term comes from the Greek root chir-, hand, and means “handedness.” Chiral molecules show a left- and right-handed nature. They exist as mirror image forms that cannot be made to overlap byrotation. Molecules showing chirality rotate the plane of polarized light. The degree of rotation is called specific rotation or optical rotation.

30 Nov 2017

Challenges with Sanger Sequencing

Sanger sequencing is a method of sequencing DNA developed by Frederick Sanger in 1977. In Sanger sequencing, chain-terminating dideoxynucleotides are incorporated into the growing DNA chain at random positions. The sequence is then inferred from the set of incrementally terminated DNA chains separated by polyacrylamide gel electrophoresis. More recently, next generation sequencing (NGS) has begun to replace Sanger sequencing, primarily because it is able to sequence millions of DNA fragments simultaneously, compared to only 384 for Sanger sequencing.

30 Nov 2017

Protein and Peptide Microarray

Protein and peptide microarrays, the emerging tools for proteomics and clinical assays, are high-throughput methods that track binding events and activities, and decide the function of proteins on a large scale. Microarrays find their place in epitope mapping and serodiagnostic applications.

29 Nov 2017

Challenges of Protein Complex Analysis

Protein complex analysis involves extensive interpretation of the structure and function of proteins, which are present in complex biological samples. Due to this, techniques like protein complex analysis have great value in understanding the complex organisms. Though recent protein complex analysis methods are efficient in identifying the structure and of protein complex, there are some limiting factors.

29 Nov 2017

Challenges in Targeted Metabolomics

Targeted metabolomics is a quantitative approach of metabolomics as it depends on measuring the known metabolites of specific pathways. Since targeted metabolomics is a continually evolving field, it faces enormous obstacles in its widespread approach.

29 Nov 2017

Clinical Biophotonics

Biophotonics is a branch of science dealing with the interaction of light in biological substances such as tissues and cells at scales ranging from microns to the nano-level. It plays a vital role in the development of healthcare services by lowering the costs of treatment, with a suitable methodology for treatment of people in the aging society. Biophotonics consists of optics, photonics, nanotechnology, and biotechnology.

28 Nov 2017

FRET: Fluorophores

Fluorophore is the specific region or structural domain of a protein that exhibits fluorescence. In any fluorescence spectroscopy, fluorophores are the most significant part to image the protein–protein interactions.

10 Nov 2017

GFP Applications

In biochemistry and cell biology, the most highly studied and developed protein is green fluorescent protein (GFP), which is derived from jellyfish Aequorea victoria. GFP has a remarkable capacity to efficiently radiate visible internal fluorophore that is extremely valuable and naturally attractive. Innovative chances are created by the high-resolution crystal structure of GFP to decipher and control the association between structure and spectroscopic function of proteins.

10 Nov 2017

Current and Future Applications of Optogenetics

Optogenetics is the science of using light to control the behavior of cells. It is one of the most rapidly evolving fields of applied research. Optogenetic techniques enable the control of electrically excitable cells such as muscle or nerve cells.

7 Nov 2017

GFP in Nature

The occurrence of bioluminescence is extremely diverse in nature. However, it is found to be rare if we calculate their presence across the entirety of the species. Although fluorescence and bioluminescence occur in aquatic and terrestrial organisms, they are widespread among aquatic organisms to attract mates, to find prey, or to protect themselves from their enemies.

6 Nov 2017

Citric Acid Cycle Regulation

The citric acid cycle is also known as the Krebs cycle or the tricarboxylic acid cycle. It is a series of reactions in a closed loop that are fundamental for cellular respiration. The citric acid cycle produces the high-energy molecule ATP (adenosine triphosphate) and byproducts that also form ATP in a further process called oxidative phosphorylation.

2 Nov 2017

Protein Purification Techniques

Protein engineering techniques are an essential part of customizing or producing proteins with specific properties which can be applied in various industrial processes. Thus they are crucial to biotechnological research.

24 Oct 2017

Nanoscience Advances in Biology

Nanoscience is the study of matter at molecular scales. That has been defined as manipulation of matter between the nanometer (nm) and micrometer (um) scale.

24 Oct 2017

Biophotonics Techniques

Biophotonics is defined as the study of biological cells, tissues, and particles with the use of light in visible and near-visible range. It plays a major role in improving the imaging and therapeutic procedures that are commonly set for clinical purposes, including advances in lasers, optics, and fluorescence detectors. There are several primary techniques used in the biophotonic study.

15 Nov 2017

Biophotonics Research

Research in the field of biophotonics is driven by several research teams in universities, university hospitals, corporate research and analysis wings, and other such research institutions.

24 Nov 2017

Biophotonics in Cell Biology Studies

Biophotonics is a branch of science where the interaction between biological substances as well as light is used for probing, manipulating and imaging cells and tissues. This emerging technique is widely used in various fields such as medicine, agriculture, and in studies related to life science and environment.

24 Nov 2017

Biophotonic Instruments

Biophotonics is the interdisciplinary science dealing with generation and utilization of light and radiant energy for detection, imaging, and manipulation of biological materials.

17 Oct 2017

Bionanotechnology Applications

Bionanotechnology is a science that sits at the convergence of nanotechnology and biology. Nanobiology and nanobiotechnology are other names that are used interchangeably with bionanotechnology. The field applies the tools of nanotechnology to biological problems, creating specialized applications.

15 Nov 2017

Bioluminescence Resonance Energy Transfer (BRET)

Bioluminescence resonance energy transfer (BRET) is a noninvasive, advanced cell-based assay technology used for detection of protein–protein interactions. BRET involves fluorescent and bioluminescent protein tags with corresponding emission and excitation properties for investigation of resonance energy transfer between the protein tags in their vicinity owing to their interactions.

13 Nov 2017