The coronavirus disease 2019 (COVID-19) pandemic has caused several waves of infections in many world regions, so strong as to overwhelm local healthcare services. In this new situation, faced with an unknown virus, the need is to produce clinical guidelines that help recognize and manage critical COVID-19. Meanwhile, conditions such as chronic obstructive pulmonary disease (COPD) are linked to a markedly increased risk of death in community pneumonia.
Study: Identification of COVID-19 and COPD common key genes and pathways using a protein-protein interaction approach. Image Credit: Siwakorn TH/ Shutterstock
*Important notice: bioRxiv publishes preliminary scientific reports that are not peer-reviewed and, therefore, should not be regarded as conclusive, guide clinical practice/health-related behavior, or treated as established information.
A new preprint available on the bioRxiv* preprint server uses computational algorithms to tease out the interrelationships between these two conditions that cause similar outcomes. Based on protein-protein interactions (PPIs), the paper shows the presence of ten genes that overlap between the two illnesses, also shared by several other deadly and debilitating diseases.
Background
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spread worldwide and has led to approximately five million deaths so far. Older adults are by far the worst affected by COVID-19, with this illness accounting for 80% of all deaths in the 65-and-above age group.
Interestingly, this age group is also at the highest risk of COPD. In this condition, found in a tenth of people over 40 years, the airflow is limited, mostly caused by chronic bronchitis or emphysema. Many genetic and environmental factors play a part in the occurrence of this disease, including irritant chemical inhalation or smoking, though only about a fifth of smokers have COPD.
While COPD patients rarely acquire COVID-19, the risk of death is much higher than non-COPD patients. Accordingly, recent recommendations have been made to update the diagnostic and treatment protocols for COPD.
Independent of COVID-19, COPD is responsible for the third-largest number of deaths in the world. Considered a polygenic condition, it renders its victims susceptible to severe disease and death following the triggering of a cytokine storm by SARS-CoV-2 infection.
The latter causes symptoms only after about five days, with death, if it occurs, taking place at an average of 14 days later, depending on the patient’s age and immunity.
However, COPD is a treatable condition, if not curable, with current therapies able to maintain a high quality of life and keep the patient safe from other respiratory illnesses. When it coexists with COVID-19, the respiratory tract suffers because of the pre-existing lung injury. Thus, severe COVID-19 is fourfold more likely in such patients.
COPD patients have higher levels of the viral entry receptor, the angiotensin-converting enzyme 2 (ACE2), as do smokers.
What did the study show?
The researchers constructed a PPI network using available data on ~5,500 COVID-19 and 296 COPD gene expression profiles to elucidate the genes involved in such complicated conditions.
They first identified 248 overlapping genes, of which ten were found to be the top common genes. These are implicated in cell-cell communication and metabolism, development, the response to stimuli, and biological regulation. These genes are mostly also involved in pathways implicated in malaria, trypanosomiasis, and inflammatory bowel disease (IBD).
The Interleukin 10 (IL 10) gene is the first common gene, or the hub gene, expressing a regulatory cytokine that modulates the inflammatory response. It is markedly upregulated in associated with the cytokine storm in severe COVID-19 patients admitted to the intensive care unit (ICU).
It is, however, low in patients with COPD who have dramatic airway inflammation. IL-10 thus serves as a marker of disease severity and is useful for monitoring treatment.
The study similarly elucidates the role of Toll-like receptor 4 (TLR4), expressed by multiple immune cells and found to be required for the initiation of inflammatory responses. When it increases excessively, hyperinflammation may set in.
The TLRs are driven by the recognition of viral RNA or dsDNA intermediate forms (called pathogen-associated molecular patterns (PAMPs). These are formed from the viral genome and recognized by pattern recognition receptors, especially the TLRs, triggering further inflammatory antiviral cascades that eventually clear the virus.
TLR4 is found to have the highest PPI with the viral spike protein compared to other TLRs. SARS-CoV-2 is found to enhance the expression of interferon-stimulated genes (ISGs) in the respiratory tract. However, the higher levels of ISG expression can cause greater ACE2 expression as well. Research shows how lung surfactants block the infection by TLR4 binding and activation.
Other hub genes include the Tumor necrosis factor (TNF), a key inflammatory mediator that is elevated in acute inflammation due to viral infection, and chronic or systemic inflammation. High TNF levels are found in patients with COPD and patients with acute COVID-19 and COPD.
Fourthly, IL6 is a potent inflammatory cytokine, with multiple actions in the inflammatory cascade. It is produced by many cell types, including epithelial airway cells and alveolar macrophages. High IL6 levels are associated with worse lung function and an accelerated rate of deterioration and skeletal muscle weakness in COPD.
IL8, also known as CXCL8, is a mediator for neutrophil tracking and is implicated in inflammatory processes, notably after viral infections. Respiratory muscle weakness in patients with COPD is traceable in part to IL8 activity, with rises in the level of this cytokine during COPD exacerbations. High CXCL8 levels are suggested to be the cause of death in severe COVID-19.
IL4 is an activator of the JAK-STAT pathway that drives inflammation and mediates hyper-responsiveness of the airways, a key COPD element.
The seventh hub gene is ICAM1, an intercellular adhesion molecule that is overproduced during early inflammation, causing the premature release of neutrophils. This molecule is elevated in COPD patients. With moderate to severe COVID-19, ICAM1 levels are high and increasing but drop in convalescence.
This may mean that these molecules are markers of COVID-19 severity. Besides, they also trigger coagulation defects. The four intracellular binding sites for SARS-CoV-2 within human cells are highly expressed in COPD patients.
The eighth hub gene is interferon-gamma (IFN-γ). This is known to be a key risk gene for COVID-19 patients with lung disease. COPD patients have a more than five times higher risk of severe COVID-19
TLR2 is the ninth hub gene, associated with a decline in lung function and evidence of inflammation in sputum, indicating its role in COPD pathogenesis and exacerbation. It is also known to recognize SARS-CoV-2 particles and may be part of the infection-pulmonary embolism pathway.
The last of the hub genes is IL18, which is thought to be tied to the abnormal inflammatory pathways in COPD. Anti-IL 18 antibodies neutralize the damage and inflammation caused by COPD in preclinical models.
What are the implications?
The current study, based on a network of PPIs, elucidated the hub genes responsible for the virulence of COVID-19. The system is complex, and the current study findings help unravel the inflammatory response to the virus. Following viral stimulation of the cytoplasmic inflammasome NLRP3 within macrophages and T helper cell type 1 (Th1), there is an increased production of IL 18 that contribute to the virulence of the infection and severity of disease,
“In summary, COPD patients with severe COVID-19 showed higher serum levels of inflammatory cytokines such as IL 2, IL 6, IL 8, IL 10, and TNF-α, indicating that the immune response may be altered globally.”
These hub genes elevated in patients with both these conditions may mean that COPD patients, who have a higher risk of severe COVID-19, need to be assessed and treated differently.
*Important notice: bioRxiv publishes preliminary scientific reports that are not peer-reviewed and, therefore, should not be regarded as conclusive, guide clinical practice/health-related behavior, or treated as established information.
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