Sponsored Content by QuanterixReviewed by Olivia FrostJun 4 2024
The Simoa® HD-X Analyzer® is the newest fully automated Simoa® bead-based immunoassay platform model. The platform has been re-engineered to provide ultra-sensitivity and dependability with 1000x greater sensitivity than traditional immunoassays.
Image Credit: Quanterix
HD-X unique capabilities
- Simple 2-step or 3-step workflow with minimum user intervention
- Built-in touch-screen control and comprehensive data analysis tools
- A diverse menu of commercially available kits
- Multiplexed detection without having to compromise on sensitivity or specificity
- Complete automation of assay incubation, wash, and data analysis steps
The ground-breaking technology of the Simoa® HD-1 Analyzer® was designed utilizing a wealth of experience. The HD-X delivers user flexibility, unparalleled sensitivity, increased productivity and best-in-class assay performance.
Simoa® isolates each bead-immunocomplex, enabling the enzyme bound to the capture antibody to generate detectable fluorescence in each well, even in the presence of a single molecule.
The analyzer provides a map of the array where each one contains more than 200,000 wells. Each well can be digitally analyzed as being either “on” (containing a target molecule) or “off.”
The proprietary Simoa® algorithm transforms the measurement to a concentration reaching femtomolar levels for most analytes. This innovative approach to digitizing ELISA produces up to a 1000-fold increase in sensitivity over other immunoassay methods.
Assay performance. Source: Quanterix
| Metric |
Simoa® HD-X |
| Sensitivity |
Up to 1000x greater sensitivity than current immunoassays |
| Dynamic Range |
>4 logs |
| Precision |
<10% CVs |
Instrument performance. Source: Quanterix
| Metric |
Simoa® HD-X |
| Throughput |
Up to 1.152 data points per shift |
| Workflow |
Batch size 1 to 288 measurements per run |
| Total Assay Time |
<2.5 hours per 96-well plate
Run Setup |
| Run Set up Time |
<30 minutes |
| Automation |
Full (sample in to data out) |
| Sample Input |
96-well plate or tubes |
| Sample Volume |
1 μl * - 100 μl |
| Instrument Footprint |
135 x 60 x 160 cm (freestanding) |
| Multiplex Capability |
Up to 6-plex |
| Assay Flexibility |
Open platform with ability to select from menu of validated assay kits or develop custom assays |
*<10 μl requires pre-dilution step
Simoa® HD-X benefits. Source: Quanterix
| . |
. |
| Ultra-sensitivity |
Up to 1000x greater sensitivity than traditional immunoassays |
| Automation |
Reproducibility and convenience of sample->answer workflow |
| Multiplexing |
Measure up to 4 biomarkers in single sample at fg/ml concentrations |
| Flexibility |
Supports both high-throughput and rapid result workflows |
| Efficiency |
Precise control of assay conditions allows for use of stored assay calibration values |
| Menu |
Fully compatible with a broad range of assays also available on HDoe-1 as well as customized homebrew assays |
| Regulatory Compliance |
Enables 21CFR Part 11 compliance with streamlined run reports and user management |
Simoa® immunoassay kits. Source: Quanterix
| . |
| Quanterix offers a broad range of complete immunoassay kits as well as a robust homebrew kit. |
| Please visit www.quanterix.com for an updated list of all available assays and for detailed specifications. |
| Contact [email protected] for assays available in prototype format and custom assay development services. |
Instrument features and benefits
Ultra-sensitivity
With up to 1000x greater sensitivity than other immunoassays, it is now possible to measure more analyte concentrations than before. This dramatic improvement in sensitivity provides new research opportunities and allows previously undetectable markers to be measured.
Very low sample volumes can be pre-diluted, preserving samples and reducing the matrix effects while still obtaining greater sensitivity than previously possible.
Automation
Running immunoassays is a mostly manual and time-consuming technique. Researchers must commit significant time to run assays or utilize additional and expensive automation systems. The Simoa® HD-X Analyzer® performs all the steps of the assay, providing increased efficiencies and consistent, precise results that minimize variabilities common with manual techniques.
Multiplexing
The Simoa® HD-X Analyzer® can measure up to 4 biomarkers in a single assay. This provides researchers with cost and sample preservation benefits without sacrificing sensitivity or precision.
Value
The Simoa® HD-X Analyzer® is a highly valuable tool for researchers, saving them time, reducing sample volumes, and enabling multiplexing. These key features offer an attractive option to those looking to increase workflow efficiency and throughput.
Precision
Thanks to Simoa’s® digital operation and automated processes, the variability, indicated by coefficients of variation (CVs), is typically less than 20 %, with many instances falling below 10 %.
Dynamic range
The proprietary Simoa algorithm uses both digital measurements at low concentrations and analog measurements at higher concentrations, allowing a remarkable dynamic range of >4 logs.
Homebrew
The Simoa® HD-X Analyzer® assists researchers in developing and optimizing their own assays by utilizing all the benefits of Simoa® on markers where pre-packaged assay kits are unavailable.
About Quanterix 
From discovery to diagnostics, Quanterix’s ultrasensitive biomarker detection is fueling breakthroughs only made possible through its unparalleled sensitivity and flexibility. Quanterix’s Simoa® technology has delivered the gold standard for earlier biomarker detection in blood, serum or plasma, with the ability to quantify proteins that are far lower than the Limit of Quantification (LoQ) of conventional analog methods.
Its industry-leading precision instruments, digital immunoassay technology and CLIA-certified Accelerator laboratory have supported research that advances disease understanding and management in neurology, oncology, immunology, cardiology and infectious disease. Quanterix has been a trusted partner of the scientific community for nearly two decades, powering research published in more than 2,500 peer-reviewed journals.
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