Dissociated Tumor Cells are single-cell suspensions obtained from solid tumors through a combination of enzymatic and mechanical dissociation methods.
These include tumor, epithelial, immune, and fibroblast cells. DTCs receive a full flow cytometry analysis of cell populations and the donor’s complete history; a board-certified pathologist reviews the source material to ensure accurate diagnosis correlation.
BioIVT possesses substantial scientific expertise in the isolation and propagation of diseased human primary cells. Primary cells from both normal and diseased cohorts provide the most accurate representation of the original tissue.
The certified pathologists verify the tissue type and diagnosis of all donor material, and the resultant cells undergo sterility testing and are known to be free of common human pathogens.
Each lot of cells comes with available donor clinical data and drug history, as well as a complete flow analysis of cell components. Specific lots are also assigned HLA typing.
Features and benefits
- Tight quality control to guarantee that the final product satisfies requirements
- Each lot’s full clinical data set is supplied
- Cryopreserved to increase convenience and give users control over their study schedule
- Sourced from the vast network of ASTERAND™ Human Tissue collection
- The availability of matched normal adjacent tissue and cells
BioIVT provides dissociated tumor cells obtained from a freshly resected tumor. Cells are suspended and cryogenically preserved. All cases come with the standard set of clinical data. BioIVT provides Dissociated Tumor Cells from multiple donors for various solid tumor indications.
Quality
Specifications
It is important to handle this material as an infectious agent. Necessary precautions should be taken. No test procedure can guarantee that the human immunodeficiency virus, hepatitis B virus, hepatitis C virus, or other infectious agents are not present.
According to the CDC/NIH handbook “Biosafety in Microbiological and Biomedical Laboratories, from Potentially Infectious Human Serum or Blood Specimens,” all blood products offered should be handled at Bio-safety Level 2.
The sole purpose of this product's sale is research and/or manufacturing. Humans and animals are not to use it, and it is forbidden to carry out additional manufacturing to produce a final product containing viable leukocytes. It is only for in vitro use. All responsibility for its use and disposal, in compliance with all regulations, rests with the user.
Each DTC lot undergoes independent screening for mycoplasma spp., bacteria, yeast, fungi, HIV-1, HIV-2, HAV, HBV, and HCV as part of BioIVT's stringent quality control procedures. To verify that each lot has a distinct profile not present in the public databases, a 9-marker STR profile is run.
After cryopreservation, each lot's viability, morphology, and growth potential are evaluated. The pathologists evaluate the resected tissue from every lot to verify the tissue type and disease condition. Every order comes with a Certificate of Analysis (COA) for each lot.