Interleukin 6 (IL-6) is a pro-inflammatory cytokine involved in bone metabolism, hematopoiesis, immune responses and embryonic development. High IL-6 is noted in patients with microbial infections and other inflammatory conditions.

Targeting IL-6 has significant clinical implications for managing, diagnosing and preventing infectious diseases.

The pro-inflammatory function of IL-6 has been controlled by a trans-signaling process involving soluble IL-6R (sIL-6R) and gp130 receptor. The production of sIL-6R is triggered by TNF-alpha and IL-1beta. These two pro-inflammatory cytokines provoke the proteolytic cleavage of IL-6R by triggering ADAM10 and ADAM17.

Dissimilar to the majority of the other soluble receptors (for example, IL-1RA and IL-18BP), sIL-6R is NOT an inhibitor of IL-6. Rather, it triggers IL-6 signaling by developing a complex with IL-6 and gp130.

Human IL-6R (Cat#: 10398-H08H) enhanced the IL-6 activity on M1 mouse myeloid leukemia cells. The ED50 is typically 20-80 ng/mL.

Human IL-6R (Cat#: 10398-H08H) enhanced the IL-6 activity on M1 mouse myeloid leukemia cells. The ED50 is typically 20-80 ng/mL. Image Credit: Sino Biological Inc

Sino Biological has come up with a panel of high-quality recombinant IL-6 and receptor proteins to aid the study of this cytokine.

Recombinant IL-6 and receptors

Sino Biological has developed bioactive recombinant IL-6 cytokines from several species, like human, rat, mouse, pig and rhesus.

Featured IL-6 protein

Human IL-6 protein: 10395-HNAE

                         High-purity                                          Low-endotoxin                                   Tag-free

Interleukin-6 (IL-6): Recombinant pro-inflammatory IL-6 and receptors           Interleukin-6 (IL-6): Recombinant pro-inflammatory IL-6 and receptors              Interleukin-6 (IL-6): Recombinant pro-inflammatory IL-6 and receptors

Image Credit: Sino Biological Inc

Bioactive recombinant IL-6 from multiple species

Table 1. Source: Sino Biological Inc

Species Cat# Activity Validated
Human 10395-HNAE Bind to Human IL-6R Cell proliferation
Mouse 50136-MNAE Cell proliferation
Rat 80076-RNAE Cell proliferation
Rhesus 90197-CNAE Cell proliferation
Sus scrofa (Pig) 62006-WNAE Bind to Human IL-6R (10398-H08H)

 

Recombinant membrane-bound IL-6 receptor: IL-6R

IL-6 illustrates restricted cross-species bioactivity. Human IL-6 has the ability to attach to both human and mouse IL-6R, but mouse IL-6 can only fix to mouse IL-6R.

Table 2. Source: Sino Biological Inc

. .
Human IL-6 Receptor 10398-H08H
Mouse IL-6 Receptor 50280-M08H
Rat IL-6 Receptor 81072-R08H
Canine IL-6 Receptor 70117-D08H
Rhesus IL-6 Receptor 90324-C08H

 

Recombinant gp130

Gp130 is a general receptor and signal transducer shared by the majority of the IL-6 family cytokines.

Such as:

  • Recombinant Rat gp130
  • Recombinant Human gp130
  • Recombinant Rhesus gp130
  • Recombinant Mouse gp130

More IL-6 family cytokines

  • IL-11
  • IL-27
  • NNT-1
  • CT-1
  • OSM
  • CNTF
  • IL-31
  • LIF

Antibodies for IL-6 and receptors

Sino Biological has developed an extensive panel of antibodies that target IL-6 cytokines and receptors. This could be utilized in WB, ELISA, IP, and IHC-P.

Neutralization antibodies: IL-6 and IL-6R

IL-6 neutralizing antibody (Cat#: 10395-R508)

Interleukin-6 (IL-6): Recombinant pro-inflammatory IL-6 and receptors

Image Credit: Sino Biological Inc

It is possible to neutralize IL-6 induced proliferation in the T1165 mouse plasmacytoma cells.

IL-6R neutralizing antibody (Cat#: 10398-MM08)

Interleukin-6 (IL-6): Recombinant pro-inflammatory IL-6 and receptors

Image Credit: Sino Biological Inc

IL-6-dependent inhibition of cell proliferation has been neutralized by Human IL-6R neutralizing Monoclonal Antibody.

ELISA kits and ELISA detection pairs for IL-6 and receptors

  • gp130 Detection Pairs
  • IL-6 Detection Pairs
  • IL-6R Detection Pairs
  • IL-6 ELISA Kit
  • IL-6R ELISA Kit
  • gp130 ELISA Kit

Other Equipment by this Supplier

While we only use edited and approved content for Azthena answers, it may on occasions provide incorrect responses. Please confirm any data provided with the related suppliers or authors. We do not provide medical advice, if you search for medical information you must always consult a medical professional before acting on any information provided.

Your questions, but not your email details will be shared with OpenAI and retained for 30 days in accordance with their privacy principles.

Please do not ask questions that use sensitive or confidential information.

Read the full Terms & Conditions.