Chronic inflammation usually involves an imbalance of pro- and anti-inflammatory cytokines. Prolonged disruptions in this balance result in the development of diseases such as osteoarthritis and rheumatoid, autoimmune disorders, inflammatory bowel diseases, and cancer, emphasizing the need to decipher the mechanisms and intercalated network of cytokines involved in chronic inflammation.
Figure 1. Cytokines involved in acute and chronic inflammatory responses (DOI:10.2741/a171). Image Credit: Sino Biological Inc.
Disturbance in levels of the key pro-inflammatory cytokines, such as IL-6, IL-1β, and TNF-α, and anti-inflammatory cytokines, such as IL-10, IL-4, and TGF-β, are mainly responsible for the development of chronic inflammation.
A number of these inflammatory mediators, such as IL-10, IL-6, TGF-β, and TNF-α, have been proven to participate in chronic inflammatory-associated tumor progression. The presence of high levels of the C-reactive protein (CRP) is one of the blood tests used to identify systemic inflammation.
Currently, cytokine therapies are used in chronic inflammatory disorder treatments but are not widely applicable. Studies are being carried out to find new and improved treatments to overcome this challenge.
Recombinant inflammatory cytokines
Sino Biological has created a comprehensive line of recombinant cytokines to aid research into chronic inflammation.
Featured recombinant cytokines
Human IL-1β protein: 10139-HNAE
Image Credit: Sino Biological Inc.
Human TNF-α protein: 10602-HNAE
Image Credit: Sino Biological Inc.
List of related recombinant cytokines (partial)
Table 1. Source: Sino Biological Inc.
| Cat# |
Species |
Molecule |
Expression Host |
Activity |
Tag |
| 67001-UNAE |
Feline |
IL-10 |
E. coli |
Active |
Native |
| 10947-HNAE |
Human |
IL-10 |
E. coli |
Active |
Native |
| 50245-MNAE |
Mouse |
IL-10 |
E. coli |
Active |
Native |
| 62000-WNAE |
Sus scrofa (Pig) |
IL-10 |
E. coli |
Active |
Native |
| 70018-DNAE |
Canine |
IL-1β |
E. coli |
Active |
Native |
| 67002-UNAE |
Feline |
IL-1β |
E. coli |
Active |
Native |
| 10139-HNAE |
Human |
IL-1β |
E. coli |
Active |
Native |
| GMP-10139-HNAE |
Human |
IL-1β |
E. coli |
Active |
Native |
| 50101-MNAE |
Mouse |
IL-1β |
E. coli |
Active |
Native |
| 65004-TNAE |
Rabbit |
IL-1β |
E. coli |
Active |
Native |
| 80023-RNAE (mature form) |
Rat |
IL-1β |
E. coli |
Active |
Native |
| 90010-CNAE |
Rhesus |
IL-1β |
E. coli |
Active |
Native |
| 62003-WNAE |
Sus scrofa (Pig) |
IL-1β |
E. coli |
Active |
Native |
| 70021-DNAE |
Canine |
IL-4 |
E. coli |
Active |
Native |
| 11846-HNAE |
Human |
IL-4 |
E. coli |
Active |
Native |
| GMP-11846-HNAE |
Human |
IL-4 |
E. coli |
Active |
Native |
| 51084-MNAE |
Mouse |
IL-4 |
E. coli |
Active |
Native |
| 10395-HNAE |
Human |
IL-6 |
E. coli |
Active |
Native |
| GMP-10395-HNAE |
Human |
IL-6 |
E. coli |
Active |
Native |
| 50136-MNAE |
Mouse |
IL-6 |
E. coli |
Active |
Native |
| 80076-RNAE |
Rat |
IL-6 |
E. coli |
Active |
Native |
| 90197-CNAE |
Rhesus |
IL-6 |
E. coli |
Active |
Native |
| 62006-WNAE |
Sus scrofa (Pig) |
IL-6 |
E. coli |
Active |
Native |
| 70087-D08H |
Canine |
TGF-β |
HEK293 Cells |
|
C-His |
| 90294-C08H |
Cynomolgus |
TGF-β |
HEK293 Cells |
Active |
C-His |
| 10804-H08H |
Human |
TGF-β |
HEK293 Cells |
Active |
C-His |
| 10804-HNAC |
Human, Rhesus, Cynomolgus, Canine |
TGF-β |
CHO Stable Cells |
Active |
Native |
| 50698-M08H-B |
Mouse |
TGF-β |
HEK293 Cells |
|
C-His |
| 80116-RNAH |
Mouse, Rat |
TGF-β |
HEK293 Cells |
Active |
Native |
| 80116-R08H |
Rat |
TGF-β |
HEK293 Cells |
|
C-His |
| 70003-DNAE |
Canine |
TNF-α |
E. coli |
Active |
Native |
| 90330-CNAE-B |
Cynomolgus |
TNF-α |
E. coli |
|
Native |
| 90018-CNAE |
Cynomolgus, Rhesus |
TNF-α |
E. coli |
Active |
Native |
| 60002-FNAE |
Ferret |
TNF-α |
E. coli |
Active |
Native |
| 10602-HNAE |
Human |
TNF-α |
E. coli |
Active |
Native |
| GMP-10602-HNAE |
Human |
TNF-α |
E. coli |
Active |
Native |
| 50349-MNAE |
Mouse |
TNF-α |
E. coli |
Active |
Native |
| 80045-RNAE |
Rat |
TNF-α |
E. coli |
Active |
Native |
Pro-inflammatory and anti-inflammatory cytokines
Pro-inflammatory cytokines
- IL-1β
- IL-6
- IL-8
- IL-9
- IL-12
- IL-15
- IL-17
- IL-18
- IFN-γ
- TNF-α
- TNF-β
Anti-inflammatory cytokines
- IL-4
- IL-10
- IL-11
- IL-13
- TGF-β
Diagnostics
Antibodies for inflammatory cytokines
Detecting inflammatory cytokines can help to identify and further delineate chronic inflammatory cascade. Sino Biological has created a panel of antibodies targeting various inflammatory cytokines with various applications in Neutralization, WB, ELISA, IHC, and FCM.
Image Credit: Sino Biological Inc.
List of related antibodies (partial)
Table 2. Source: Sino Biological Inc.
| Cat# |
Species |
Antigen |
Application |
Clonality |
Label |
| 10947-MM07-B |
Human |
IL-10 |
ELISA |
Mouse MAb |
Biotin |
| 10947-MM19-B |
Human |
IL-10 |
ELISA |
Mouse MAb |
Biotin |
| 10947-R128-B |
Human |
IL-10 |
ELISA |
Rabbit MAb |
Biotin |
| 10139-M201 |
Human |
IL-1β |
ELISA, IHC-P |
Mouse MAb |
|
| 50101-R001 |
Mouse |
IL-1β |
ELISA |
Rabbit MAb |
|
| 80023-MM71 |
Rat |
IL-1β |
ELISA |
Mouse MAb |
|
| 80023-R312 |
Rat |
IL-1β |
WB,ELISA |
Rabbit MAb |
|
| 11846-MM01-H |
Human |
IL-4 |
ELISA |
Mouse MAb |
HRP |
| 11846-MM04-B |
Human |
IL-4 |
ELISA |
Mouse MAb |
Biotin |
| 11846-MM04-H |
Human |
IL-4 |
ELISA |
Mouse MAb |
HRP |
| 11846-R404 |
Human |
IL-4 |
WB |
Rabbit MAb |
|
| 51084-R015 |
Mouse |
IL-4 |
ELISA |
Rabbit MAb |
|
| 10395-MK23 |
Human |
IL-6 |
Neutralization |
Mouse MAb |
|
| 10395-MM10 |
Human |
IL-6 |
IHC-P |
Mouse MAb |
|
| 10395-MM19 |
Human |
IL-6 |
WB, IHC-P |
Mouse MAb |
|
| 10395-MM72-B |
Human |
IL-6 |
ELISA |
Mouse MAb |
Biotin |
| 10395-MM72-H |
Human |
IL-6 |
ELISA |
Mouse MAb |
HRP |
| 10395-R508 |
Human |
IL-6 |
Neutralization |
Rabbit MAb |
|
| 50136-R076 |
Mouse |
IL-6 |
ELISA |
Rabbit MAb |
|
| 80076-R007 |
Rat |
IL-6 |
ELISA |
Rabbit MAb |
|
| 10804-MM33 |
Human |
TGF-β |
WB |
Mouse MAb |
|
| 10804-R016-H |
Human |
TGF-β |
ELISA |
Rabbit MAb |
HRP |
| 10602-MM01-F |
Human |
TNF-α |
FCM |
Mouse MAb |
FITC |
| 10602-MM08-B |
Human |
TNF-α |
ELISA |
Mouse MAb |
Biotin |
| 10602-MM08-H |
Human |
TNF-α |
ELISA |
Mouse MAb |
HRP |
| 10602-MM0N1 |
Human |
TNF-α |
Neutralization |
Mouse MAb |
|
| 10602-R101-A |
Human |
TNF-α |
FCM |
Rabbit MAb |
APC |
| 10602-R101-F |
Human |
TNF-α |
FCM |
Rabbit MAb |
FITC |
| 10602-R101-P |
Human |
TNF-α |
FCM |
Rabbit MAb |
PE |
| 10602-R10N1 |
Human |
TNF-α |
FCM, Neutralization |
Rabbit MAb |
|
| 10602-RE23-B |
Human |
TNF-α |
ELISA |
Rabbit MAb |
Biotin |
| 50349-R009 |
Mouse |
TNF-α |
ELISA |
Rabbit MAb |
|
| 50349-RN023 |
Mouse |
TNF-α |
Neutralization |
Rabbit MAb |
|
| 60002-R303 |
Ferret |
TNF-α |
ELISA (Cap) |
Rabbit MAb |
|
| 70003-R001 |
Canine |
TNF-α |
ELISA |
Rabbit MAb |
|
| 80045-R141 |
Rat |
TNF-α |
WB, ICC/IF |
Rabbit MAb |
|