Sponsored Content by AbcamMay 2 2019
Below is a list of high impact products for studies on the cyclin-dependent kinase (CDK) inhibitor p21 (also known as WAF1, CAP20, Cip1, and Sdi1). The tables show the most-cited research targets and widely-referenced products. Browse to understand better the effect of p21 in cellular function.
The p21 Toolkit
| Type |
Reagent name |
Description |
AbID |
Primary
antibody |
Anti-p21 antibody
|
Type: Rabbit monoclonal antibody
Reactivity: Hu
Applications: ICC/IF, Flow Cyt, WB, IP, IHC-P |
ab109520 |
Conjugated
primary
antibody |
Anti-p21 antibody |
Biotin conjugated |
ab79467 |
Recommended
secondaries |
Goat Anti-Rabbit IgG H&L |
Alexa Fluor® 488 conjugated |
ab150077 |
| HRP conjugated |
ab205718 |
| IRDye® 800CW (preadsorbed) |
ab216773 |
| Kit |
p21 SimpleStep ELISA® kit |
Quantitatively measure p21 in human cell and tissue extracts. Get fast, reproducible results in 90 minutes.
Reactivity: Hu
Range: 62.5 pg/ml - 4000 pg/ml
Method: Colorimetric |
ab214658 |
| Protein |
Recombinant p21 protein |
Human p21 protein for use in WB and as control1. |
ab56278 |
| Small molecule compound |
UC2288 (inhibitor)
*NEW* |
Selective p21 inhibitor structurally related to Sorafenib (ab141966). Inhibits cytosolic p21 (IC50 = 10 µM) and functions independently of p532,3. |
ab146969 |
| Tenovin-1 (activator) |
p21 activator and inducer of cell cycle arrest4. |
ab120889 |
Activation of p53-p21 Pathway
DNA damaging agents activate the p53-p21 pathway causing cell cycle arrest and DNA repair5. This mechanism is exploited by chemotherapeutic agents that induce p21 as a means of inhibiting cancer progression. Investigating p21 regulation in a p53-dependent or -independent manner can form a main basis of tumor suppression6,7.
| Reagent |
Type |
Purpose |
AbID |
| Oxaliplatin |
Inducer, inhibitor |
Exhibits dual role in p53-dependent and -independent pathways. Causes G0-G1 arrest in p53 wild type cells and p21 degradation in p53 mutants8. |
ab141054 |
| Nocodazole |
Inducer |
p21 inducer that increases expression of p21 in a p53-dependent fashion in absence of DNA damage9,10. |
ab120630 |
| Cycloheximide (CHX) |
Modulator |
Induction of p21 during serum stimulation (indicating serum contains a p21 blocking element)7. CHX traditionally acts as a p21 inhibitor promoting apoptosis in serum-starved cells11. |
ab120093 |
| PMA |
Inducer |
p53-independent p21 induction via Sp1 binding sites within p21 promoter12. |
ab120297 |
| Okadaic acid |
ab120375 |
References
- Sun, Z. et al. Increased SHP-1 expression results in radioresistance, inhibition of cellular senescence, and cell cycle redistribution in nasopharyngeal carcinoma cells. Radiat. Oncol. 10, 152 (2015).
- Wettersten, H. I. et al. A novel p21 attenuator which is structurally related to sorafenib. Cancer Biol. Ther. 14, 278–285 (2013).
- Liu, R., Wettersten, H. I., Park, S.-H. & Weiss, R. H. Small-molecule inhibitors of p21 as novel therapeutics for chemotherapy-resistant kidney cancer. Future Med. Chem. 5, 991–4 (2013).
- Wilking, M. J., Singh, C., Nihal, M., Zhong, W. & Ahmad, N. SIRT1 deacetylase is overexpressed in human melanoma and its small molecule inhibition imparts anti-proliferative response via p53 activation. Arch. Biochem. Biophys. 563, 94–100 (2014).
- He, G. et al. Induction of p21 by p53 following DNA damage inhibits both Cdk4 and Cdk2 activities. Oncogene 24, 2929–2943 (2005).
- Galanos, P. et al. Chronic p53-independent p21 expression causes genomic instability by deregulating replication licensing. Nat. Cell Biol. 18, 777 (2016).
- Macleod, K. F. et al. p53-dependent and independent expression of p21 during cell growth, differentiation, and DNA damage. Genes Dev. 9, 935–44 (1995).
- Hata, T. et al. Role of p21waf1/cip1 in effects of oxaliplatin in colorectal cancer cells. Mol. Cancer Ther. 4, 1585–1594 (2005).
- Lanni, J. S. & Jacks, T. Characterization of the p53-Dependent Postmitotic Checkpoint following Spindle Disruption. Mol. Cell. Biol. 18, 1055–1064 (1998).
- Linke, S. P., Clarkin, K. C., Di Leonardo, A., Tsou, A. & Wahl, G. M. A reversible, p53-dependent G0/G1 cell cycle arrest induced by ribonucleotide depletion in the absence of detectable DNA damage. Genes Dev. 10, 934–947 (1996).
- Arbel-Goren, R., Levy, Y., Ronen, D. & Zick, Y. Cyclin-dependent kinase inhibitors and JNK act as molecular switches, regulating the choice between growth arrest and apoptosis induced by galectin-8. J. Biol. Chem. 280, 19105–19114 (2005).
- Kennett, S. Sp3 encodes multiple proteins that differ in their capacity to stimulate or repress transcription. Nucleic Acids Res. 25, 3110–3117 (1997).
About Abcam
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