Analyzing biopharmaceutical processes including cell growth in bioreactors

Raman spectroscopy has proven its ability to accurately detect subtle changes in complex aqueous systems, greatly broadening its application scope, including monitoring biopharmaceutical processes like cell growth in bioreactors.

Utilized as a continuous process analyzer, it enables the non-destructive, real-time, in-situ monitoring of complex chemical systems during biopharmaceutical production. This capability to track shifts in various metabolites throughout bioreactor operations has solidified its role as a valuable Process Analytical Technology (PAT) tool.

One of the key advantages of Raman Spectroscopy is the use of reusable analysis optical probes, which enhance process repeatability and reliability by minimizing variability between runs. The Thermo Scientific™ MarqMetrix All-in-One Process Raman Analyzer offers a wide selection of optical probes to suit different needs.

This article delves into the integration of the MarqMetrix All-in-One Process Raman Analyzer with the 500L DynaDrive Single-Use bioreactor for inline measurement of critical process parameters (CPPs). The combined system facilitates accurate predictive modeling for various parameters and metabolites, marking a significant advancement in process monitoring.

For further details on how this integrated approach enhances predictive accuracy and process control, download the complete article here.

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About Thermo Fisher Scientific – Portable and Handheld Raman Spectroscopy

Thermo Fisher Scientific offers innovative solutions that help our customers solve complex analytical challenges, accelerate life sciences manufacturing, deliver medicines to market, and increase laboratory productivity. Our Thermo Scientific portable and handheld process Raman analyzers enable accurate, real-time results for process monitoring. 

The Thermo Scientific™ MarqMetrix™ All-In-One Process Raman Analyzer is an all-in-one system purpose-built for rapid deployment, ease of use, and scalability in markets where time-to-results is critical. The MarqMetrix All-In-One Process Raman Analyzer is designed for:

  • Analysis without sample preparation, delivering Raman spectral results in real-time
  • Easy setup and deployment by non–Raman spectroscopists
  • Non-destructive workflows to protect precious samples
  • Non-invasive handling to minimize contamination of samples
  • Small footprint for convenient deployment
  • Factory calibration for hardware stability and portability

The Thermo Scientific TruScan™Handheld Raman Analyzers includes state-of-the-art optics paired with a patented multivariate residual analysis that offers an effective chemometric solution for material identification, with two spectral pre-processing options. The non-destructive point-and-shoot sampling principle facilitates rapid verification of a broad range of chemical compounds, including cellulose-based products.

The TruScan™ G3 Handheld Raman Analyzer continues the evolution of raw material ID testing for pharmaceutical and biotechnology manufacturing quality control processes with:

  • Incoming raw material identification (RMID)
  • Dispensing of materials during API manufacture
  • Finished product inspection
  • Identification of falsified (counterfeit) medicines
  • Enables non-technical operators to rapidly perform raw material ID testing without the need of a lab

The TruScan™ RM Handheld Raman Analyzer takes pharmaceutical manufacturing QA/QC to the next level with: 

  • Enhanced 21 CFR Part 11 and cGMP compliance with biometric login, complex password options, and full audit trail features
  • Non-contact analysis through plastic bags, glass containers, blister packs and clear gel caps
  • Intuitive workflow adapted to production environment through PDF batch reports and ease of data input using barcode scanners
  • Easy fleet management feature that enables cloning of instruments and identification methods

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Last updated: May 27, 2024 at 2:58 AM

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