ExtraClean kits for RNA purification and exosome isolation

ExtraClean Kits for RNA Purification and Exosome Isolation offer advanced solutions for RNA purification, concentration, and exosome isolation.

Key features

• Achieve optimal results for sensitive applications such as NGS
• Enhance microRNA mapping by up to tenfold during sequencing runs, reducing costs

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EXTRAClean RNA Clean-Up and Concentration Micro-Elute Kit

Norgen’s EXTRAClean RNA Clean-Up and Concentration Micro-Elution Kit provides a rapid method for the purification, cleanup, and concentration of up to 10 μg of RNA isolated using different methods.

Image Credit: Norgen Biotek Corporation

Format

Micro-Elute

Size

50 Preps 

Features and benefits

• Assure the best outcomes for delicate applications such as NGS.
• MicroRNA mapping during sequencing runs could be increased by up to ten times to cut expenses.
• Small-scale RNA concentration into 8 μL
• Perfect for concentrating RNA extracted from exosomes, plasma, serum, urine, and other body fluids, as well as any RNA samples that were first purified in large quantities.
• Effective RNA removal from enzymatic processes: in vitro transcription, DNase treatment, and labeling
• Cleanup of RNA that has been isolated using various techniques, such as phenol/chloroform extractions
• Processing in the rapid spin-column format is quick and simple.
• Appropriate for RNA of all sizes, ranging from microRNA (miRNA) to large rRNA
• No extractions of phenol or chloroform
• EXTRAClean spin column chromatography, which uses Norgen's exclusive separation matrix, is the basis for purification.

A quick way to purify, clean up, and concentrate up to 10 μg of RNA extracted using phenol/guanidine-based protocols and from a variety of upstream enzymatic reactions, including DNase treatment, labeling, and in vitro transcription, is offered by Norgen's EXTRAClean RNA Clean-Up and Concentration Micro-Elution Kit. It is possible to concentrate small amounts of RNA of all sizes, from large mRNA and ribosomal RNA to microRNA (miRNA) and small interfering RNA (siRNA), using an elution volume of at least 8 μL.

Without the use of phenol or chloroform, RNA is preferentially separated from other reaction constituents like proteins, RNases, and nucleotides. To reduce contamination traces and guarantee the best results for delicate applications like NGS, the EXTRAClean columns undergo a rigorous processing and quality control procedure.

RNase protection and primer extension, Northern blotting, end-point or quantitative reverse transcription PCR, expression array assays, and next-generation sequencing are just a few of the downstream uses for purified RNA, which has the highest integrity.

Details

Source: Norgen Biotek Corporation

Storage conditions and product stability

Every solution needs to be stored at room temperature and kept tightly sealed. This kit remains stable for two years following the date of shipment.

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EXTRAClean Plasma/Serum Exosome Purification and RNA Isolation Kits

Norgen’s Plasma/Serum Exosome and RNA Isolation Kits provide an all-in-one solution for exosome purification and sequential exosomal RNA isolation from plasma/serum samples. After exosome purification, they enable the separation of exosomal RNA from sample volumes up to 10 mL.

Format

Mini

Image Credit: Norgen Biotek Corporation

Size

50 Preps

Midi

Image Credit: Norgen Biotek Corporation

Size

25 Preps

Maxi

Image Credit: Norgen Biotek Corporation

Size

15 Preps

Features and benefits

• Make sure sensitive applications like NGS get the best results possible.
• MicroRNA mapping during sequencing runs can be increased up to tenfold to reduce costs.
• Exosomes from intact plasma or serum are purified and enriched for functional research.
• Variable volume of plasma or serum input.
• No carrier RNA, Proteinase K treatment, or phenol extractions are needed.
• There is no need for time-consuming filtration, ultracentrifugation, or specialized syringes.
• Neither an overnight incubation period nor precipitation reagents are needed.
• Suitable for serum or plasma from any species.
• Pure exosomes are devoid of any additional RNA-binding proteins.
• Using Norgen's exclusive resin separation matrix, EXTRAClean Mini Spin Column chromatography serves as the basis for purification.
• Small RNA sequencing is one of the many downstream uses for the purified RNA that are appropriate.

Norgen’s EXTRAClean Plasma/Serum Exosome Purification and RNA Isolation Mini Kit is an all-in-one system for exosome purification and subsequent exosomal RNA isolation from plasma/serum sample volumes ranging from 50 μL to 1 mL. The purification process uses Norgen's proprietary resin in spin column chromatography. To reduce contamination traces and guarantee the best results for delicate applications like NGS, the EXTRAClean columns undergo a rigorous processing and quality control procedure.

The kit separates extracellular vesicle RNA of all sizes, including microRNA. It has a distinct advantage over other kits on the market because it does not require any specialized equipment, extension tubes, protein precipitation reagents, phenol/chloroform, or protease treatments. The kit enables the user to elute into a variable elution volume between 50 and 100 µL.

The purified RNA has the highest integrity and is devoid of any circulating RNA bound to proteins. Numerous downstream processes, such as real-time PCR, NGS application, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays, can use the purified RNA.

Details

Supporting data

Average read quality distribution relative to raw reads of small RNA sequencing of exosomal RNA extracted from plasma and serum samples using EXTRAClean Plasma/Serum Exosome Purification and RNA Isolation Mini Kit. Calculations were relative to raw reads. Image Credit: Norgen Biotek Corporation

Genome mapping distribution relative to input reads obtained from small RNA sequencing of exosomal RNA extracted from various plasma and serum samples using EXTRAClean Plasma/Serum Exosome Purification and RNA Isolation Mini Kit. Image Credit: Norgen Biotek Corporation

Average small RNA biotype distribution relative to the input reads obtained from sequencing of exosomal RNA extracted from various plasma and serum using EXTRAClean Plasma/Serum Exosome Purification and RNA Isolation Mini Kit. Image Credit: Norgen Biotek Corporation

Average read quality distribution relative to raw reads of small RNA sequencing of exosomal RNA extracted from plasma and serum samples using EXTRAClean Plasma/Serum Exosome Purification and RNA Isolation Midi Kit. Calculations were relative to raw reads. Image Credit: Norgen Biotek Corporation

Genome mapping distribution relative to input reads obtained from small RNA sequencing of exosomal RNA extracted from various plasma and serum samples using EXTRAClean Plasma/Serum Exosome Purification and RNA Isolation Midi Kit. Image Credit: Norgen Biotek Corporation

Average small RNA biotype distribution relative to the input reads obtained from sequencing of exosomal RNA extracted from various plasma and serum using EXTRAClean Plasma/Serum Exosome Purification and RNA Isolation Midi Kit. Image Credit: Norgen Biotek Corporation

Genome mapping distribution relative to input reads obtained from small RNA sequencing of exosomal RNA extracted from various plasma and serum samples using EXTRAClean Plasma/Serum Exosome Purification and RNA Isolation Maxi Kit. Image Credit: Norgen Biotek Corporation

Average small RNA biotype distribution relative to the input reads obtained from sequencing of exosomal RNA extracted from various plasma and serum using EXTRAClean Plasma/Serum Exosome Purification and RNA Isolation Maxi Kit. Image Credit: Norgen Biotek Corporation

Source: Norgen Biotek Corporation

Storage conditions and product stability

All solutions should be stored tightly sealed at room temperature. This kit remains stable for up to two years after the shipment date. If salt precipitation occurs in Lysis Buffer A, warm it at 60 °C for 20 minutes before use.

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EXTRAClean Exosomal RNA Isolation Kit

EXTRAClean Exosomal RNA Isolation Kit is designed for the isolation of exosomal RNA from exosomes previously purified using Norgen's Exosome Purification Kits or Norgen’s EXTRAClean Exosome Purification Kits.

Image Credit: Norgen Biotek Corporation

Format

Spin Column

Size

50 Preps

Features and benefits

• Ensure optimal results for sensitive applications such as NGS
• Achieve up to a tenfold increase in microRNA mapping during sequencing runs, reducing costs.
• Isolates all sizes of exosomal and extracellular vesicle RNA, including microRNA.
• Bind and elute all RNA without bias, regardless of size or GC content.
• No phenol extractions
• No Proteinase K treatment
• No carrier RNA
• Concentrate the extracted RNA into an elution volume that is flexible and can range from 50 μL to 100 μL.
• High-quality RNA can be purified in 15 to 20 minutes.
• Purified RNA can be used for small RNA sequencing and other downstream processes.
• EXTRAClean spin column chromatography, which makes use of Norgen's exclusive separation matrix, is the basis for purification.

The kit is made to separate RNA of all sizes, including microRNA. The kit has a distinct advantage over other kits on the market because it does not require specialized equipment, extension tubes, protein precipitation reagents, phenol/chloroform, or protease treatments.

The kit enables the user to elute into a variable elution volume between 50 and 100 microliters. RNase protection and primer extension, Northern blotting, real-time PCR, reverse transcription PCR, and expression array assays are just a few of the downstream uses for purified RNA, which has the highest integrity.

Details

Supporting data

Genome mapping distribution relative to input reads obtained from small RNA sequencing of exosomal RNA extracted from various plasma and serum samples using EXTRAClean Plasma/Serum Exosome Purification and RNA Isolation Maxi Kit. Image Credit: Norgen Biotek Corporation

Average small RNA biotype distribution relative to the input reads obtained from sequencing of exosomal RNA extracted from various plasma and serum using EXTRAClean Plasma/Serum Exosome Purification and RNA Isolation Maxi Kit. Image Credit: Norgen Biotek Corporation

Average read quality distribution relative to raw reads of small RNA sequencing of exosomal RNA extracted from various urine volumes using EXTRAClean Urine Exosome Purification and RNA Isolation Maxi Kit. Calculations were relative to raw reads. Image Credit: Norgen Biotek Corporation

Genome mapping distribution relative to input reads obtained from small RNA sequencing of exosomal RNA extracted from various urine volumes using EXTRAClean Urine Exosome Purification and RNA Isolation Maxi Kit. Image Credit: Norgen Biotek Corporation

Average small RNA biotype distribution relative to the input reads obtained from sequencing of exosomal RNA extracted from various urine volumes using EXTRAClean Urine Exosome Purification and RNA Isolation Maxi Kit. Image Credit: Norgen Biotek Corporation

Source: Norgen Biotek Corporation

Storage conditions and product stability

Every buffer needs to be stored at room temperature and kept tightly sealed. For two years following the date of shipment, this kit remains stable. If any salt precipitation is seen, Lysis Buffer A should be warmed for 20 minutes at 60 °C.

Download the Infographic for More Information