Rapid and reliable DNA isolation from saliva samples

The Saliva DNA Isolation Kit efficiently extracts high-quality DNA from fresh and preserved saliva samples.

Image Credit: Norgen Biotek Corp.

Image Credit: Norgen Biotek Corp.

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Format

Spin column

Size

50 preps

Overview

The Saliva DNA Isolation Kit is a rapid and simple spin column process for isolating genomic DNA from saliva samples collected and conserved using Norgen’s Saliva DNA Collection and Preservation Devices (Cat. RU49000) or fresh saliva samples.

PCR, Southern Blot analysis, sequencing, and microarray analysis are just a few of the downstream research applications that can use the highest-quality saliva DNA that has been purified using Norgen’s kit.

Features and benefits

• Processing in a rapid spin-column format is quick and simple
• High-quality genomic DNA can be extracted from preserved or fresh saliva samples
• Compatible with preserved saliva samples collected using Norgen’s Saliva DNA Collection and Preservation Devices (Cat. RU49000)

Background

Saliva is an effective noninvasive alternative to blood collection. Human genomic DNA isolated from buccal epithelial cells and white blood cells in saliva has a variety of diagnostic applications. Saliva DNA can be used to detect biomarkers to diagnose a disease, track its progression, or monitor the effects of a specific treatment. Saliva DNA can also be used to identify some forms of diseases.

Isolation of DNA from saliva has become a popular alternative to isolation from blood or tissue since it is non-invasive, can be collected by anybody with no training, and does not require any specific equipment. Norgen's spit DNA Isolation Kit is a quick and easy way to isolate genomic DNA from both preserved and fresh spit samples.

Details

Supporting data

Figure 1. High Quality and Yield of DNA from Saliva Samples. Total DNA was isolated from 250 µL of eight different fresh saliva samples using Norgen's Saliva DNA Isolation Kit (Lanes 1-8). For evaluation, 10 µL of each 100 µL DNA elution was run on a 1.2 % agarose gel. Note the high yield and quality of the DNA in all lanes. Lane M: Norgen's Fast runner 1kb DNA Ladder. Image Credit: Norgen Biotek Corp.

Figure 2. Real-time PCR Consistency from Saliva Samples. Norgen's Saliva DNA Isolation Kit was used to isolate DNA from 250 µL fresh saliva samples. Ten samples were used from same donor. From the 100 µL elution, 5 µL of saliva DNA was directly mixed to real-time PCR master mix (total reaction volume 20 µL), and the real-time PCR reaction was performed. The GAPDH gene was successfully amplified from the different samples without any inhibition and at the same Ct, indicating the isolation consistency and the excellent quality of saliva DNA for downstream applications. Image Credit: Norgen Biotek Corp.

Figure 3. Illumina MiSeq 16s rRNA metagenomics data from saliva samples preserved for over 6 years using Norgen's Saliva DNA Collection and Preservation Devices. The saliva DNA was isolated using Norgen's Saliva DNA Isolation Kit (Cat. RU45400) from saliva that had been preserved for various periods of time up to 6 years at room temperature. A) Principal Coordinate Analysis of 12 saliva microbiomes showing differences in the distribution of taxonomic classifications between samples up to kingdom level. B) Hierarchical clustering of 12 saliva microbiomes based on genus-level classifications, including a bar chart showing the relative abundance of genus-level classifications for each sample in the dendrogram. Image Credit: Norgen Biotek Corp.

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Kit specifications

Source: Norgen Biotek Corp.

* Average yield will depending on the donor

Storage conditions and product stability

All solutions should be tightly packed and stored at room temperature. This kit is stable for one year from the date of dispatch. The package includes a ready-to-use Proteinase K dissolved in a carefully designed storage buffer. When stored at room temperature, buffered Proteinase K will remain stable for up to a year after shipment.

Source: Norgen Biotek Corp.