Total RNA purification from soil samples

Soil Total RNA Purification Kit from Norgen Biotek is a fast and efficient solution for extracting high-quality, inhibitor-free total RNA from soil samples.

Format

Spin Column

Size

50 Preps

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Features and benefits

• Extract superior total RNA from a range of soil samples
• Handle all kinds of soil, such as manure, compost, and regular soil
• Eliminate all traces of humic acids and additional PCR inhibitors
• Removes phenol from RNA of all sizes, including microRNA
• Complete kit with humic acid removal columns (HAR) and bead tubes
• Spin column chromatography, which makes use of Norgen's exclusive resin separation matrix, is the basis for purification

This kit offers a quick and easy way to purify total RNA from tiny amounts of soil samples using a spin column. This kit can be used to process any kind of soil sample, including common soil samples and challenging soil samples like compost and manure that have a high humic acid content. Using humic acid removal (HAR) columns, the kit eliminates all traces of humic acids and other PCR inhibitors.

Without the use of phenol, Norgen's Soil Total RNA Purification Kit purifies RNA of all sizes, from large mRNA and ribosomal RNA to microRNA and small interfering RNA. For any downstream application, such as microbiome studies, real-time PCR and reverse transcription PCR for gene expression analysis, Next Generation Sequencing, and more, the purified RNA has the highest integrity and is free of inhibitors.

Details

Supporting data

Figure 1. Isolation of Total RNA from Bacteria in Soil. Pseudomonas fluorescens was spiked into 250 mg samples of autoclaved soil and total RNA was isolated using Norgen's Soil Total RNA Purification Kit. RNA was visualized by running 7.5 µL of each 75 µL elution on a 1.2% agarose-formaldehyde RNA gel. Total RNA (large and small) of Pseudomonas fluorescens was recovered from the autoclaved spiked soil without any significant degradation, indicating that high integrity RNA can be purified from the microorganisms in the soil. Lanes 1 and 2 contain total RNA from Pseudomonas fluorescens, Lanes 3 and 4 contain total RNA purified from the autoclaved soil spiked with Pseudomonas fluorescens, and Lanes 5 and 6 contain RNA purified from the autoclaved soil (no RNA was found). Image Credit: Norgen Biotek Corporation

Figure 2. High Quality RNA Free from PCR Inhibitors. Pseudomonas fluorescens was spiked into 250 mg samples of autoclaved soil and total RNA was isolated using Norgen's Soil Total RNA Purification Kit. One microliter of each elution was then used as the template in a 20 µL RT-PCR reaction to detect the 16s rRNA. Lane 1 contains the results when total RNA from Pseudomonas fluorescens was used as the input, Lane 2 is the results when total RNA isolated from the autoclaved soil spiked with Pseudomonas fluorescens was used as the input, Lane 3 is the results when RNA purified from non-spiked autoclaved soil was the input, Lane 4 is a positive control and Lane 5 is the Negative control. As it can be seen the RNA purified from soil using Norgen's kit was of a high quality and can be successfully used in sensitive downstream applications. Image Credit: Norgen Biotek Corporation

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Kit specifications

Source: Norgen Biotek Corporation

Storage conditions and product stability

All solutions should be tightly sealed and stored at room temperature. The kit remains stable for one year from the shipment date.

Source: Norgen Biotek Corporation